TY - CHAP
T1 - D Glucose enhancement of water reabsorption in proximal tubule of the rat kidney
AU - Weinman, E. J.
AU - Suki, Wadi N.
AU - Eknoyan, G.
PY - 1976/12/1
Y1 - 1976/12/1
N2 - Water reabsorption in the proximal convoluted tubule of the rat kidney was examined by in vivo microperfusion techniques in order to examine the effect of D glucose within the tubular lumen. When tubules were perfused with a balanced artificial solution containing Na, K, Cl, HCO3, urea, and D glucose, absolute reabsorption averaged 4.01±0.24 nl/min per mm tubule length. Perfusion with isotonic NaCl resulted in a 37% decrease in reabsorption of 2.54±0.17 nl/min per mm. Addition of D glucose to the NaCl perfusate enhanced water reabsorption to values similar to those obtained with the balanced artificial perfusate. The enhanced water reabsorption consequent to the addition of D glucose to the NaCl perfusion solution was completely inhibited by addition of phloridzin to the perfusate. The addition of an unabsorbed hexose, 2 deoxy D glucose, to the NaCl perfusate failed to enhance water reabsorption, whereas the addition of an incompletely reabsorbed sugar that is not metabolized, 3 O methyl D glucose, resulted in partial enhancement of the absolute rate of water reabsorption. These studies demonstrate that D glucose has the specific effect of augmenting water reabsorption in the proximal tubule of the rat kidney.
AB - Water reabsorption in the proximal convoluted tubule of the rat kidney was examined by in vivo microperfusion techniques in order to examine the effect of D glucose within the tubular lumen. When tubules were perfused with a balanced artificial solution containing Na, K, Cl, HCO3, urea, and D glucose, absolute reabsorption averaged 4.01±0.24 nl/min per mm tubule length. Perfusion with isotonic NaCl resulted in a 37% decrease in reabsorption of 2.54±0.17 nl/min per mm. Addition of D glucose to the NaCl perfusate enhanced water reabsorption to values similar to those obtained with the balanced artificial perfusate. The enhanced water reabsorption consequent to the addition of D glucose to the NaCl perfusion solution was completely inhibited by addition of phloridzin to the perfusate. The addition of an unabsorbed hexose, 2 deoxy D glucose, to the NaCl perfusate failed to enhance water reabsorption, whereas the addition of an incompletely reabsorbed sugar that is not metabolized, 3 O methyl D glucose, resulted in partial enhancement of the absolute rate of water reabsorption. These studies demonstrate that D glucose has the specific effect of augmenting water reabsorption in the proximal tubule of the rat kidney.
UR - http://www.scopus.com/inward/record.url?scp=0017172301&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017172301&partnerID=8YFLogxK
M3 - Chapter
C2 - 970456
AN - SCOPUS:0017172301
VL - 231
SP - 777
EP - 780
BT - American Journal of Physiology
ER -