Abstract
Tumor-targeted fluorescent dyes have been shown to significantly improve a surgeon's ability to locate and resect occult malignant lesions, thereby enhancing a patient's chances of long term survival. Although several tumor-targeted fluorescent dyes have been developed for imaging specific subsets of human cancers, no tumor-targeted dye has been designed that can image all cancer types. Based on observations that fibroblast activation protein (FAP) is upregulated on cancer-associated fibroblasts (CAFs) that infiltrate essentially all solid tumors, we have undertaken to develop a FAP-targeted fluorescent dye that can image CAFs without accumulating in healthy cells or fibroblasts. We report here that FTL-S-S0456, a novel FAP-targeted near infrared dye that binds FAP with high affinity (∼12 nM) and specificity (>5000-fold over PREP and DPP-IV), concentrates in all seven solid tumor types examined, yielding fluorescence images with high tumor to background ratios that persist for several days. We conclude that FTL-S-S0456 constitutes an excellent ligand-targeted near infrared dye that enables intra-operative imaging of most if not all solid tumors.
Original language | English (US) |
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Pages (from-to) | 2038-2046 |
Number of pages | 9 |
Journal | Journal of Materials Chemistry B |
Volume | 10 |
Issue number | 12 |
DOIs | |
State | Published - Mar 23 2022 |
Keywords
- Cell Line, Tumor
- Fibroblasts/metabolism
- Fluorescence
- Fluorescent Dyes/metabolism
- Humans
- Neoplasms/diagnostic imaging
- Proteins
ASJC Scopus subject areas
- Chemistry(all)
- Materials Science(all)
- Biomedical Engineering