TY - JOUR
T1 - Development of a protocol to assess cell internalization and tissue uptake of magnetic nanoparticles by AC Biosusceptometry
AU - Quini, Caio C.
AU - Próspero, André G.
AU - Kondiles, Bethany R.
AU - Chaboub, Lesley
AU - Hogan, Matthew K.
AU - Baffa, Oswaldo
AU - Bakuzis, Andris F.
AU - Horner, Philip J.
AU - Miranda, José R.A.
N1 - Funding Information:
The authors acknowledge the following funding institutions: Fundação de Amparo à Pesquisa do Estado de São Paulo ( FAPESP ), grant number 2015/14914-0 and 2017/00842-2 . Brazilian Federal Agency for Support and Evaluation of Graduate Education within the Ministry of Education of Brazil (Capes).
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Several applications of nanoparticles rely on the internalization and accumulation of nanocarriers in specific cell compartments and tissues. However, the methods currently employed for characterizing such processes, although well described, are time consuming and do not provide in vivo information, which is a crucial barrier towards translational applications. Here, we hypothesize that the AC Biosusceptometry technique can be employed to assess cell internalization of magnetic nanoparticles, with possible applications in screening assays to track specific biomarkers and cell types. We tested a simpler and easier alternative to study cell internalization and tissue accumulation after perfusion. We utilized citrate coated, manganese ferrite nanoparticles and evaluated the internalization process in mouse macrophages cells (J774.A1) and in an embryonic neural stem cell culture (E14.5) after differentiation in astrocytes and neurons, to assess internalization specificity. Respecting the particles toxicity limits, we tested different concentration of particles, in different incubation times. Sequentially, we imaged the cell cultures to confirm internalization and nanoparticles localization, labeling nucleus and cell body to assure that the particles were inside the cells. Our results showed a linear behavior on internalization for different doses and an optimum incubation time of 2 h.
AB - Several applications of nanoparticles rely on the internalization and accumulation of nanocarriers in specific cell compartments and tissues. However, the methods currently employed for characterizing such processes, although well described, are time consuming and do not provide in vivo information, which is a crucial barrier towards translational applications. Here, we hypothesize that the AC Biosusceptometry technique can be employed to assess cell internalization of magnetic nanoparticles, with possible applications in screening assays to track specific biomarkers and cell types. We tested a simpler and easier alternative to study cell internalization and tissue accumulation after perfusion. We utilized citrate coated, manganese ferrite nanoparticles and evaluated the internalization process in mouse macrophages cells (J774.A1) and in an embryonic neural stem cell culture (E14.5) after differentiation in astrocytes and neurons, to assess internalization specificity. Respecting the particles toxicity limits, we tested different concentration of particles, in different incubation times. Sequentially, we imaged the cell cultures to confirm internalization and nanoparticles localization, labeling nucleus and cell body to assure that the particles were inside the cells. Our results showed a linear behavior on internalization for different doses and an optimum incubation time of 2 h.
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U2 - 10.1016/j.jmmm.2018.10.064
DO - 10.1016/j.jmmm.2018.10.064
M3 - Article
AN - SCOPUS:85056565340
SN - 0304-8853
VL - 473
SP - 527
EP - 533
JO - Journal of Magnetism and Magnetic Materials
JF - Journal of Magnetism and Magnetic Materials
ER -