TY - JOUR
T1 - Differences in removal of acetylaminofluorene and pyrimidine dimers from the DNA of cultured mammalian cells
AU - Amacher, D. E.
AU - Elliott, J. A.
AU - Lieberman, M. W.
PY - 1977
Y1 - 1977
N2 - The rate and extent of disappearance of 2 DNA lesions (pyrimidine dimers and covalently bound acetylaminofluorene), both thought to be removed by the socalled wide patch (approximately 100 nucleotides) repair process, were studied in a variety of cultured mammalian cells. With the exception of mouse cells, dimers were removed more rapidly and extensively than covalently bound acetylaminofluorene. In human cells, for example, about 50% of the dimers were excised from DNA in 1 hr while only 25 to 50% of the chemically induced lesions were excised from DNA after 48 hr. Surprisingly mouse cells, which remove few dimers, were about as competent as control human fibroblasts at removing acetylaminofluorene lesions; however, xeroderma pigmentosum cells (group D) removed fewer N acetoxy 2 acetylaminofluorene induced lesions than control human cells. The data raise the possibility of separate repair processes for these 2 types of lesions and suggest that their expression may be under similar genetic control in human cells.
AB - The rate and extent of disappearance of 2 DNA lesions (pyrimidine dimers and covalently bound acetylaminofluorene), both thought to be removed by the socalled wide patch (approximately 100 nucleotides) repair process, were studied in a variety of cultured mammalian cells. With the exception of mouse cells, dimers were removed more rapidly and extensively than covalently bound acetylaminofluorene. In human cells, for example, about 50% of the dimers were excised from DNA in 1 hr while only 25 to 50% of the chemically induced lesions were excised from DNA after 48 hr. Surprisingly mouse cells, which remove few dimers, were about as competent as control human fibroblasts at removing acetylaminofluorene lesions; however, xeroderma pigmentosum cells (group D) removed fewer N acetoxy 2 acetylaminofluorene induced lesions than control human cells. The data raise the possibility of separate repair processes for these 2 types of lesions and suggest that their expression may be under similar genetic control in human cells.
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U2 - 10.1073/pnas.74.4.1553
DO - 10.1073/pnas.74.4.1553
M3 - Article
C2 - 266196
AN - SCOPUS:0017618314
SN - 0027-8424
VL - 74
SP - 1553
EP - 1557
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -