@inproceedings{09821d22bf8a43679af7a27d1b98367e,
title = "Differentiating quiescent cancer cell populations in heterogeneous samples with fluorescence lifetime imaging",
abstract = "Measurement of relative fluorescence intensities of NAD(P)H and FAD with fluorescence lifetime imaging (FLIM) allows metabolic characterization of cancerous populations and correlation to treatment response. However, quiescent populations of cancer cells introduce heterogeneity to the tumor and exhibit resistance to standard therapies, requiring a better understanding of this influence on treatment outcome. Significant differences were observed between proliferating and quiescent cell populations upon comparison of respective redox ratios (p<0.05) and FAD lifetimes (p<0.05) across monolayers and in mixed samples. These results demonstrate that metabolic activity may function as a marker for separation and characterization of proliferating and quiescent cancer cells within mixed samples, contributing to comprehensive investigation of heterogeneity-dependent drug resistance.",
keywords = "cancer, cellular heterogeneity, fluorescence lifetime, metabolic imaging, quiescence",
author = "Heaster, {Tiffany M.} and Walsh, {Alex J.} and Skala, {Melissa C.}",
note = "Funding Information: Funding sources include the NIH R01-CA185747 and NSF GRFP DGE-0909667. Publisher Copyright: {\textcopyright} 2016 SPIE.; Biophysics, Biology, and Biophotonics: The Crossroads ; Conference date: 13-02-2016 Through 14-02-2016",
year = "2016",
doi = "10.1117/12.2211734",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Adam Wax and Vadim Backman",
booktitle = "Biophysics, Biology, and Biophotonics",
address = "United States",
}