TY - JOUR
T1 - DNA sensor-associated type I interferon signaling is increased in ulcerative colitis and induces JAK-dependent inflammatory cell death in colonic organoids
AU - Flood, Peter
AU - Fanning, Aine
AU - Woznicki, Jerzy A.
AU - Crowley, Tadhg
AU - Christopher, Andrea
AU - Vaccaro, Alessandra
AU - Houston, Aileen
AU - McSweeney, Sheila
AU - Ross, Sarah
AU - Hogan, Aileen
AU - Brint, Elizabeth
AU - Skowyra, Agnieszka
AU - Bustamante, Milan
AU - Ambrose, Monica
AU - Moloney, Gerard
AU - MacSharry, John
AU - Hammarström, Marie Louise
AU - Hurley, Margot
AU - Fitzgibbons, Christine
AU - Quigley, Eamonn M.M.
AU - Shanahan, Fergus
AU - Zulquernain, Syed A.
AU - McCarthy, Jane
AU - Steven Dodson, G.
AU - Dabbagh, Karim
AU - McRae, Bradford L.
AU - Melgar, Silvia
AU - Nally, Ken
N1 - Funding Information:
K.N. and F.S. received research funding from AbbVie Inc. as part of a research centre spoke award (SFI-14/SP/2710) to APC
Funding Information:
This work was supported by Science Foundation Ireland; namely, a research center grant (SFI-12/RC/2273, to P. Ross) and a research center spoke award (SFI-14/SP/2710, to K. Nally and F. Shanahan) to APC Microbiome Ireland. In addition, part of this work was supported by separate research funding from Second Genome to APC Microbiome Ireland.
Publisher Copyright:
Copyright © 2022 the American Physiological Society.
PY - 2022/11
Y1 - 2022/11
N2 - DNA sensor pathways can initiate inflammasome, cell death, and type I interferon (IFN) signaling in immune-mediated inflammatory diseases (IMIDs), including type I interferonopathies. We investigated the involvement of these pathways in the pathogenesis of ulcerative colitis (UC) by analyzing the expression of DNA sensor, inflammasome, and type I IFN biomarker genes in colonic mucosal biopsy tissue from control (n = 31), inactive UC (n = 31), active UC (n = 33), and a UC single-cell RNA-Seq dataset. The effects of type I IFN (IFN-β), IFN-γ, and TNF-α on gene expression, cytokine production, and cell death were investigated in human colonic organoids. In organoids treated with cytokines alone, or in combination with NLR family pyrin domain-containing 3 (NLRP3), caspase, or JAK inhibitors, cell death was measured, and supernatants were assayed for IL-1β/IL-18/CXCL10. The expression of DNA sensor pathway genes-PYHIN family members [absent in melanoma 2 (AIM2), IFI16, myeloid cell nuclear differentiation antigen (MNDA), and pyrin and HIN domain family member 1 (PYHIN1)- as well as Z-DNA-binding protein 1 (ZBP1), cyclic GMP-AMP synthase (cGAS), and DDX41 was increased in active UC and expressed in a cell type-restricted pattern. Inflammasome genes (CASP1, IL1B, and IL18), type I IFN inducers [stimulator of interferon response cGAMP interactor 1 (STING), TBK1, and IRF3), IFNB1, and type I IFN biomarker genes (OAS2, IFIT2, and MX2) were also increased in active UC. Cotreatment of organoids with IFN-β or IFN-γ in combination with TNFα increased expression of IFI16, ZBP1, CASP1, cGAS, and STING induced cell death and IL-1β/IL-18 secretion. This inflammatory cell death was blocked by the JAK inhibitor tofacitinib but not by inflammasome or caspase inhibitors. Increased type I IFN activity may drive elevated expression of DNA sensor genes and JAK-dependent but inflammasome-independent inflammatory cell death of colonic epithelial cells in UC.
AB - DNA sensor pathways can initiate inflammasome, cell death, and type I interferon (IFN) signaling in immune-mediated inflammatory diseases (IMIDs), including type I interferonopathies. We investigated the involvement of these pathways in the pathogenesis of ulcerative colitis (UC) by analyzing the expression of DNA sensor, inflammasome, and type I IFN biomarker genes in colonic mucosal biopsy tissue from control (n = 31), inactive UC (n = 31), active UC (n = 33), and a UC single-cell RNA-Seq dataset. The effects of type I IFN (IFN-β), IFN-γ, and TNF-α on gene expression, cytokine production, and cell death were investigated in human colonic organoids. In organoids treated with cytokines alone, or in combination with NLR family pyrin domain-containing 3 (NLRP3), caspase, or JAK inhibitors, cell death was measured, and supernatants were assayed for IL-1β/IL-18/CXCL10. The expression of DNA sensor pathway genes-PYHIN family members [absent in melanoma 2 (AIM2), IFI16, myeloid cell nuclear differentiation antigen (MNDA), and pyrin and HIN domain family member 1 (PYHIN1)- as well as Z-DNA-binding protein 1 (ZBP1), cyclic GMP-AMP synthase (cGAS), and DDX41 was increased in active UC and expressed in a cell type-restricted pattern. Inflammasome genes (CASP1, IL1B, and IL18), type I IFN inducers [stimulator of interferon response cGAMP interactor 1 (STING), TBK1, and IRF3), IFNB1, and type I IFN biomarker genes (OAS2, IFIT2, and MX2) were also increased in active UC. Cotreatment of organoids with IFN-β or IFN-γ in combination with TNFα increased expression of IFI16, ZBP1, CASP1, cGAS, and STING induced cell death and IL-1β/IL-18 secretion. This inflammatory cell death was blocked by the JAK inhibitor tofacitinib but not by inflammasome or caspase inhibitors. Increased type I IFN activity may drive elevated expression of DNA sensor genes and JAK-dependent but inflammasome-independent inflammatory cell death of colonic epithelial cells in UC.
KW - DNA sensors
KW - colonic organoids
KW - inflammasome
KW - type I IFN
KW - ulcerative colitis
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U2 - 10.1152/ajpgi.00104.2022
DO - 10.1152/ajpgi.00104.2022
M3 - Article
C2 - 36165492
AN - SCOPUS:85140856974
SN - 0193-1857
VL - 323
SP - G439-G460
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 5
ER -