Abstract
The rapid transmission and resilience of coronavirus disease 2019 (COVID-19) have led to urgent demands in monitoring humoral response for effective vaccine development, thus a multiplex co-detection platform to discriminate infection-induced from vaccine-induced antibodies is needed. Here a duplex electrochemical immunosensor for co-detection of anti-nucleocapsid IgG (N-IgG) and anti-spike IgG (S-IgG) is developed by using a two-working electrode system, via an indirect immunoassay, with antibody quantification obtained by differential pulse voltammetry. The screen-printed electrodes (SPEs) are modified by carbon black and electrodeposited gold nanoflowers for maximized surface areas, enabling the construction of an immunological chain for S-IgG and N-IgG electrochemical detection with enhanced performance. Using an optimized immunoassay protocol, a wide linear range between 30–750 and 20–1000 ng mL−1, and a limit of detection of 28 and 15 ng mL−1 are achieved to detect N-IgG and S-IgG simultaneously in serum samples. This duplex immunosensor is then integrated in a microfluidic device to obtain significantly reduced detection time (≤ 7 min) while maintaining its analytical performance. The duplex microfluidic immunosensor can be easily expanded into multiplex format to achieve high throughput screening for the sero-surveillance of COVID-19 and other infectious diseases.
Original language | English (US) |
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Article number | 2207731 |
Pages (from-to) | e2207731 |
Journal | Small |
Volume | 19 |
Issue number | 51 |
DOIs | |
State | Published - Dec 20 2023 |
Keywords
- SARS-CoV-2 antibody detection
- dual immunosensors
- microfluidics
- screen-printed electrodes
- vaccine development
- Gold
- Immunoassay/methods
- Humans
- Immunoglobulin G
- Vaccines
- COVID-19/diagnosis
- Antibodies, Viral
- Electrodes
- Biosensing Techniques
- Electrochemical Techniques/methods
- Microfluidics
ASJC Scopus subject areas
- Engineering (miscellaneous)
- Chemistry(all)
- Materials Science(all)
- Biotechnology
- Biomaterials