TY - JOUR
T1 - ECM-based macroporous sponges release essential factors to support the growth of hematopoietic cells
AU - Soffer-Tsur, Neta
AU - Peer, Dan
AU - Dvir, Tal
N1 - Funding Information:
TD acknowledges partial support from the Israel Science Foundation Individual Grant 700/13. The work is part of the doctoral thesis of NST.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/7/10
Y1 - 2017/7/10
N2 - The success of hematopoietic stem cells (HSCs) transplantation is limited due to the low number of HSCs received from donors. In vivo, HSCs reside within a specialized niche inside the 3D porous spongy bone. The natural environment in the niche is composed of structural proteins, glycosaminoglycans (GAGs) and soluble factors that control cells fate. However, the designed scaffolds for in vitro culture do not fairly recapitulate this microenvironment and cannot efficiently control HSCs fate. Here we report on the development of new omental ECM-based 3D macroporous sponges for hematopoietic cell culture. The scaffolds' structure, porosity and stability were characterized and optimized. Analysis of the biochemical content revealed that they were composed of collagens and GAGs, including sulfated GAGs. This morphology and composition enabled growth factors interaction with the sulfated GAGs, as indicated by the high loading capacity and release profile of three different hematopoietic niche factors. Finally, the ability of the ECM-based scaffolds to efficiently support the growth of hematopoietic cells by releasing stem cell factor (SCF) was demonstrated.
AB - The success of hematopoietic stem cells (HSCs) transplantation is limited due to the low number of HSCs received from donors. In vivo, HSCs reside within a specialized niche inside the 3D porous spongy bone. The natural environment in the niche is composed of structural proteins, glycosaminoglycans (GAGs) and soluble factors that control cells fate. However, the designed scaffolds for in vitro culture do not fairly recapitulate this microenvironment and cannot efficiently control HSCs fate. Here we report on the development of new omental ECM-based 3D macroporous sponges for hematopoietic cell culture. The scaffolds' structure, porosity and stability were characterized and optimized. Analysis of the biochemical content revealed that they were composed of collagens and GAGs, including sulfated GAGs. This morphology and composition enabled growth factors interaction with the sulfated GAGs, as indicated by the high loading capacity and release profile of three different hematopoietic niche factors. Finally, the ability of the ECM-based scaffolds to efficiently support the growth of hematopoietic cells by releasing stem cell factor (SCF) was demonstrated.
KW - Hematopoietic cells
KW - Scaffolds
KW - Tissue engineering
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U2 - 10.1016/j.jconrel.2016.09.021
DO - 10.1016/j.jconrel.2016.09.021
M3 - Article
C2 - 27671876
AN - SCOPUS:85002245199
SN - 0168-3659
VL - 257
SP - 84
EP - 90
JO - Journal of Controlled Release
JF - Journal of Controlled Release
ER -