Effect of hypothermic preservation on biological tissues in physiological solution

M. G. Ghosn; M. A. Mohamed; A. Mehralizad; M. Mashiatulla; S. Syed; F. Castro-Chavez; J. D. Morrisett; K. V. Larin

doi:10.1117/12.875114

Effect of hypothermic preservation on biological tissues in physiological solution

M. G. Ghosn, M. A. Mohamed, A. Mehralizad, M. Mashiatulla, S. Syed, F. Castro-Chavez, J. D. Morrisett, K. V. Larin

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Preservation of porcine aortic tissue at 4°C in phosphate buffered saline (PBS) was monitored for a period of 10 days. Optical coherence tomography (OCT) was used to indirectly quantify the permeation of glucose, with the objective of assessing the structural integrity of the tissue. The average permeability rate for the first day was calculated to be (2.32 ± 0.46) × 10^-5 cm/s. After 10 days of storage the average permeability rate was found to be (7.37 ± 0.41) × 10^-5 cm/s-nearly a 200% increase. A z-test performed on the permeability rate results verified that after 4 days of storage the permeability rate had significantly changed (p<0.05). Histology was used to validate the OCT results by quantifying changes in pore area. An increase in pore size paralleled the increase in permeability rate over the 10 day storage period. A parallel experiment demonstrated that increasing pore size was not accompanied by release of protein from the tissue over the storage period. The results suggest that tissues can maintain their structural stability for at least three days at 4°C in PBS.

Original language	English (US)
Title of host publication	Dynamics and Fluctuations in Biomedical Photonics VIII
DOIs	https://doi.org/10.1117/12.875114
State	Published - 2011
Event	Dynamics and Fluctuations in Biomedical Photonics VIII - San Francisco, CA, United States Duration: Jan 22 2011 → Jan 24 2011

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7898
ISSN (Print)	1605-7422

Conference

Conference	Dynamics and Fluctuations in Biomedical Photonics VIII
Country/Territory	United States
City	San Francisco, CA
Period	1/22/11 → 1/24/11

Keywords

Cold tissue storage
Hypothermic preservation
Optical coherence tomography (OCT)
Permeability rate
Porcine aorta
Pore size morphology

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.875114

Cite this

Ghosn, M. G., Mohamed, M. A., Mehralizad, A., Mashiatulla, M., Syed, S., Castro-Chavez, F., Morrisett, J. D., & Larin, K. V. (2011). Effect of hypothermic preservation on biological tissues in physiological solution. In Dynamics and Fluctuations in Biomedical Photonics VIII Article 789806 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7898). https://doi.org/10.1117/12.875114

Effect of hypothermic preservation on biological tissues in physiological solution. / Ghosn, M. G.; Mohamed, M. A.; Mehralizad, A. et al.
Dynamics and Fluctuations in Biomedical Photonics VIII. 2011. 789806 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7898).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ghosn, MG, Mohamed, MA, Mehralizad, A, Mashiatulla, M, Syed, S, Castro-Chavez, F, Morrisett, JD & Larin, KV 2011, Effect of hypothermic preservation on biological tissues in physiological solution. in Dynamics and Fluctuations in Biomedical Photonics VIII., 789806, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7898, Dynamics and Fluctuations in Biomedical Photonics VIII, San Francisco, CA, United States, 1/22/11. https://doi.org/10.1117/12.875114

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abstract = "Preservation of porcine aortic tissue at 4°C in phosphate buffered saline (PBS) was monitored for a period of 10 days. Optical coherence tomography (OCT) was used to indirectly quantify the permeation of glucose, with the objective of assessing the structural integrity of the tissue. The average permeability rate for the first day was calculated to be (2.32 ± 0.46) × 10-5 cm/s. After 10 days of storage the average permeability rate was found to be (7.37 ± 0.41) × 10-5 cm/s-nearly a 200% increase. A z-test performed on the permeability rate results verified that after 4 days of storage the permeability rate had significantly changed (p<0.05). Histology was used to validate the OCT results by quantifying changes in pore area. An increase in pore size paralleled the increase in permeability rate over the 10 day storage period. A parallel experiment demonstrated that increasing pore size was not accompanied by release of protein from the tissue over the storage period. The results suggest that tissues can maintain their structural stability for at least three days at 4°C in PBS.",

keywords = "Cold tissue storage, Hypothermic preservation, Optical coherence tomography (OCT), Permeability rate, Porcine aorta, Pore size morphology",

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Effect of hypothermic preservation on biological tissues in physiological solution

Abstract

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Keywords

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