Fiber bundle based endomicroscopy prototype with two collection channels for simultaneous coherent anti-Stokes Raman scattering and second harmonic generation imaging

Zhengfan Liu, Zachary A. Satira, Xi Wang, Xiaoyun Xu, Xu Chen, Kelvin Wong, Shufen Chen, Jianguo Xin, Stephen T C Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Label-free multiphoton imaging is promising for replacing biopsy and could offer new strategies for intraoperative or surgical applications. Coherent anti-Stokes Raman scattering (CARS) imaging could provide lipid-band contrast, and second harmonic generation (SHG) imaging is useful for imaging collagen, tendon and muscle fibers. A combination of these two imaging modalities could provide rich information and this combination has been studied by researchers to investigate diseases through microscopy imaging. The combination of these two imaging modalities in endomicroscopy imaging has been rarely investigated. In this research, a fiber bundle consisted of one excitation fiber and 18 collection fibers was developed in our endomicroscopy prototype. The 18 collection fibers were divided into two collection channels with 9 fibers in each channel. These two channels could be used together as one channel for effective signal collection or used separately for simplifying detection part of the system. Differences of collection pattern of these two channels were investigated. Collection difference of central excitation fiber and surrounding 18 fibers was also investigated, which reveals the potential ability of this system to measure forward to backward (F/B) ratio in SHG imaging. CARS imaging of mouse adipocyte and SHG imaging of mouse tail tendon were performed to demonstrate the CARS and SHG tissue imaging performance of this system. Simultaneous CARS and SHG imaging ability of this system was demonstrated by mouse tail imaging. This fiber bundle based endomicroscopy imaging prototype, offers a promising platform for constructing efficient fiber-based CARS and SHG multimodal endomicroscopes for label free intraoperative imaging applications.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIV
PublisherSPIE
ISBN (Print)9780819498618
DOIs
StatePublished - 2014
EventMultiphoton Microscopy in the Biomedical Sciences XIV - San Francisco, CA, United States
Duration: Feb 2 2014Feb 4 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8948
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences XIV
Country/TerritoryUnited States
CitySan Francisco, CA
Period2/2/142/4/14

Keywords

  • coherent anti-Stokes Raman scattering
  • endomicroscopy
  • endoscopy
  • fiber optics
  • multimodal imaging
  • nonlinear optics
  • second harmonic generation

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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