Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases

Qiang Wang; Leiyun Weng; Hongbing Jiang; Shijian Zhang; Tetsuya Toyoda

doi:10.1016/j.ab.2012.10.025

Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases

Qiang Wang, Leiyun Weng, Hongbing Jiang, Shijian Zhang, Tetsuya Toyoda

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

Original language	English (US)
Pages (from-to)	92-94
Number of pages	3
Journal	Analytical Biochemistry
Volume	433
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2012.10.025
State	Published - Feb 15 2013

Keywords

Enterovirus 71
Fluorescein isothiocyanate
Hepatitis C virus
In vitro transcription
Influenza virus
Japanese encephalitis virus

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2012.10.025

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title = "Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases",

abstract = "Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.",

keywords = "Enterovirus 71, Fluorescein isothiocyanate, Hepatitis C virus, In vitro transcription, Influenza virus, Japanese encephalitis virus",

author = "Qiang Wang and Leiyun Weng and Hongbing Jiang and Shijian Zhang and Tetsuya Toyoda",

note = "Funding Information: This work was supported by Grants-in-Aid from the Chinese Academy of Sciences ( 0514P51131 and KSCX1-YW-10 ), the Chinese 973 Project ( 2009CB522504 ), the Chinese National Science and Technology Major Project ( 2008ZX10002-014 ), and the Li Ka-Shing Foundation ( 0682P11131 ). ",

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AU - Wang, Qiang

AU - Weng, Leiyun

AU - Jiang, Hongbing

AU - Zhang, Shijian

AU - Toyoda, Tetsuya

N1 - Funding Information: This work was supported by Grants-in-Aid from the Chinese Academy of Sciences ( 0514P51131 and KSCX1-YW-10 ), the Chinese 973 Project ( 2009CB522504 ), the Chinese National Science and Technology Major Project ( 2008ZX10002-014 ), and the Li Ka-Shing Foundation ( 0682P11131 ).

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N2 - Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

AB - Viral infection is a leading cause of disease and death. Although vaccines are the most effective method of controlling viral infections, antiviral drugs are also important. Here, we established an in vitro transcription system by using fluorescein isothiocyanate-conjugated primers for RNA polymerases of viruses that are important disease-causing human pathogens (influenza, hepatitis C, Japanese encephalitis viruses, and enterovirus 71). This technology will allow us to analyze RNA polymerase activity without using radioisotopes.

KW - Enterovirus 71

KW - Fluorescein isothiocyanate

KW - Hepatitis C virus

KW - In vitro transcription

KW - Influenza virus

KW - Japanese encephalitis virus

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Fluorescent primer-based in vitro transcription system of viral RNA-dependent RNA polymerases

Abstract

Keywords

ASJC Scopus subject areas

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