Abstract
Dendritic cells play a pivotal role in host immune defense, such as elimination of foreign pathogen and inhibition of tumorigenesis. In this paper, we report that [Gd@C82(OH)22]n could induce phenotypic maturation of dendritic cells by stimulating DC production of cytokines including IL-12p70, upregulating DC co-stimulatory (CD80, CD83, and CD86) and MHC (HLA-A,B,C and HLA-DR) molecules, and switching DCs from a CCL5-responsive to a CCL19-responsive phenotype. We found that [Gd@C 82(OH)22]n can induce dendritic cells to become functionally mature as illustrated by their capacity to activate allogeneic T cells. Mice immunized with ovalbumin in the presence of [Gd@C 82(OH)22]n exhibit enhanced ovalbumin-specific Th1-polarized immune response as evidenced by the predominantly increased production of IFN-, IL-1-, and IL-2. The [Gd@C82(OH) 22]n nanoparticle is a potent activator of dendritic cells and Th1 immune responses. These new findings also provide a rational understanding of the potent anticancer activities of [Gd@C82(OH) 22]n nanoparticles reported previously.
Original language | English (US) |
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Pages (from-to) | 1178-1186 |
Number of pages | 9 |
Journal | ACS Nano |
Volume | 4 |
Issue number | 2 |
DOIs | |
State | Published - Feb 23 2010 |
Keywords
- Activation
- Dendritic cells
- Fullerene
- Nanomedicine
- Th1 polarization
ASJC Scopus subject areas
- Engineering(all)
- Materials Science(all)
- Physics and Astronomy(all)