Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Duan Jinliang; Bai Fang; Zhu Xiaofeng; He Xiaoshun; Hu Anbin

doi:10.1016/j.scr.2023.103302

Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Duan Jinliang, Bai Fang, Zhu Xiaofeng, He Xiaoshun, Hu Anbin

Research output: Contribution to journal › Article › peer-review

Abstract

CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.

Original language	English (US)
Article number	103302
Pages (from-to)	103302
Journal	Stem Cell Research
Volume	75
Early online date	Jan 5 2024
DOIs	https://doi.org/10.1016/j.scr.2023.103302
State	Published - Mar 2024

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1016/j.scr.2023.103302

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title = "Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential",

abstract = "CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.",

author = "Duan Jinliang and Bai Fang and Zhu Xiaofeng and He Xiaoshun and Hu Anbin",

note = "Funding Information: This work was financially supported by the National Natural Science Foundation of China (No. 82070635, 81672998, 81901514) and Natural Science Foundation of Guangdong province of China (No. 2019A1515011364). Publisher Copyright: {\textcopyright} 2024 The Authors",

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T1 - Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

AU - Jinliang, Duan

AU - Fang, Bai

AU - Xiaofeng, Zhu

AU - Xiaoshun, He

AU - Anbin, Hu

N1 - Funding Information: This work was financially supported by the National Natural Science Foundation of China (No. 82070635, 81672998, 81901514) and Natural Science Foundation of Guangdong province of China (No. 2019A1515011364). Publisher Copyright: © 2024 The Authors

PY - 2024/3

Y1 - 2024/3

N2 - CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.

AB - CCL22 is a macrophage-derived immunosuppressive chemokine that recruits regulatory T cells through the CCL22:CCR4 axis, playing an important role in homeostatic and inflammatory responses. A CCL22-overexpressing human induced pluripotent stem cell line (CNNDi001-A-2) was generated by lentiviral transduction to further study the function of CCL22. The cell line was confirmed to have normal proliferation and pluripotency and could be further differentiated into islet cells for cell replacement therapy in diabetes.

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Generation of a human induced pluripotent stem cell line overexpressing CCL22 with islet cells differentiation potential

Abstract

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