Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity

Tarrik Zaid; Trivandrum Sukumaran Nair Srikumar; Ludmil Benov

doi:10.5483/bmbrep.2003.36.6.608

Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity

Tarrik Zaid, Trivandrum Sukumaran Nair Srikumar, Ludmil Benov

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a Δfur mutant The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.

Original language	English (US)
Pages (from-to)	608-610
Number of pages	3
Journal	Journal of Biochemistry and Molecular Biology
Volume	36
Issue number	6
DOIs	https://doi.org/10.5483/bmbrep.2003.36.6.608
State	Published - Nov 30 2003

Keywords

Catalase activity
Fur
Hydroperoxidase I
Iron

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.5483/bmbrep.2003.36.6.608

Cite this

@article{6ad8bb09edf84a5f9a7342f9cb19ff9b,

title = "Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity",

abstract = "Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a Δfur mutant The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.",

keywords = "Catalase activity, Fur, Hydroperoxidase I, Iron",

author = "Tarrik Zaid and Srikumar, {Trivandrum Sukumaran Nair} and Ludmil Benov",

year = "2003",

month = nov,

day = "30",

doi = "10.5483/bmbrep.2003.36.6.608",

language = "English (US)",

volume = "36",

pages = "608--610",

journal = "Journal of Biochemistry and Molecular Biology",

issn = "1225-8687",

publisher = "Springer Verlag",

number = "6",

}

TY - JOUR

T1 - Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity

AU - Zaid, Tarrik

AU - Srikumar, Trivandrum Sukumaran Nair

AU - Benov, Ludmil

PY - 2003/11/30

Y1 - 2003/11/30

N2 - Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a Δfur mutant The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.

AB - Escherichia coli has two catalases, HPI and HPII. HPI is induced during logarithmic growth in response to low concentrations of hydrogen peroxide. This induction is OxyR-dependent. On the other hand, HPII is not peroxide-inducible but is induced in entry to the stationary phase. We demonstrate here that E. coli displayed higher HPI catalase activity when compared to the cultures that were grown in a normal medium, if grown in a medium supplemented with iron-citrate. Iron supplementation had no effect on HPII catalase. This increase of HPI activity was OxyR-independent and not observed in a Δfur mutant The physiological significance of the increase of HPI activity is unclear, but it appears that the katG gene that codes for HPI catalase is among the genes that are regulated by Fur.

KW - Catalase activity

KW - Fur

KW - Hydroperoxidase I

KW - Iron

UR - http://www.scopus.com/inward/record.url?scp=0942268090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0942268090&partnerID=8YFLogxK

U2 - 10.5483/bmbrep.2003.36.6.608

DO - 10.5483/bmbrep.2003.36.6.608

M3 - Article

C2 - 14659082

AN - SCOPUS:0942268090

SN - 1225-8687

VL - 36

SP - 608

EP - 610

JO - Journal of Biochemistry and Molecular Biology

JF - Journal of Biochemistry and Molecular Biology

IS - 6

ER -

Growth of Escherichia coli in Iron-enriched Medium Increases HPI Catalase Activity

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this