TY - JOUR
T1 - HBO1 directs histone H4 specific acetylation, potentiating mechano-transduction pathways and membrane elasticity in ovarian cancer cells
AU - Quintela, Marcos
AU - Sieglaff, Douglas H.
AU - Gazze, Andrea Salvatore
AU - Zhang, Aijun
AU - Gonzalez, Deyarina
AU - Francis, Lewis
AU - Webb, Paul
AU - Conlan, R. Steven
N1 - Funding Information:
Funding: This work was supported by the Natural and Environmental Research Council (NERC), UK [grant number NE/K004212/1]; Houston Methodist Research Institute; and Swansea University.
Publisher Copyright:
© 2019 The Author(s)
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/4
Y1 - 2019/4
N2 - New approaches to treat ovarian cancer, the fifth leading cause of cancer mortality among women, are being sought, with the targeting of epigenetic modulators now receiving much attention. The histone acetyltransferase HBO1 functions in regulating diverse molecular processes, including DNA repair, transcription and replication, and is highly expressed in primary ovarian cancer. Here we define both the molecular function and a role in cell biomechanics for HBO1 in ovarian cancer. HBO1 preferentially acetylates histone H4 through the concomitant overexpression of co-regulator JADE2, and is required for the expression of YAP1, an ovarian cancer oncogene and mechano-transductor signaling factor. HBO1 appears therefore to have a role in determining the mechano-phenotype in ovarian cancer cells, through both signal transduction processes, and the modulation of cell elasticity as observed using direct measurements on live cells via atomic force microscopy.
AB - New approaches to treat ovarian cancer, the fifth leading cause of cancer mortality among women, are being sought, with the targeting of epigenetic modulators now receiving much attention. The histone acetyltransferase HBO1 functions in regulating diverse molecular processes, including DNA repair, transcription and replication, and is highly expressed in primary ovarian cancer. Here we define both the molecular function and a role in cell biomechanics for HBO1 in ovarian cancer. HBO1 preferentially acetylates histone H4 through the concomitant overexpression of co-regulator JADE2, and is required for the expression of YAP1, an ovarian cancer oncogene and mechano-transductor signaling factor. HBO1 appears therefore to have a role in determining the mechano-phenotype in ovarian cancer cells, through both signal transduction processes, and the modulation of cell elasticity as observed using direct measurements on live cells via atomic force microscopy.
KW - Acetyltransferase
KW - Biomechanics
KW - Elasticity
KW - JADE
KW - YAP1
UR - http://www.scopus.com/inward/record.url?scp=85062703578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062703578&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2019.01.017
DO - 10.1016/j.nano.2019.01.017
M3 - Article
C2 - 30759370
AN - SCOPUS:85062703578
SN - 1549-9634
VL - 17
SP - 254
EP - 265
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
ER -