High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices

Zhongcheng Gong; Fang Nie; Tianhua Zhang; Pushparaj Pathak; Zhiyong Wang; Kemi Cui; Hong Zhao; Stephen Wong; Long Que

doi:10.1109/MEMSYS.2010.5442381

High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices

Zhongcheng Gong, Fang Nie, Tianhua Zhang, Pushparaj Pathak, Zhiyong Wang, Kemi Cui, Hong Zhao, Stephen Wong, Long Que

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

This paper reports the analysis of drug effects on single breast cancer cells using the droplet-microfluidic devices for the first time. Hundreds of droplets can be generated in less than 5 minutes. Each droplet serves as a nanoliter-volume titer plate. About 50% of the droplets contain a human breast cancer cell MDA-MB-231 and Cytochalasin D drug solution. The drug effects on each cell are monitored by analyzing the fluorescence image of each cell. Clear change of the cell shape and size has been observed after incubation, suggesting this approach is a potential viable technical platform for drug effect analysis and for high throughput drug screen and discovery.

Original language	English (US)
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	1015-1018
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442381
State	Published - 2010
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - Hong Kong, China Duration: Jan 24 2010 → Jan 28 2010

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Country/Territory	China
City	Hong Kong
Period	1/24/10 → 1/28/10

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

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10.1109/MEMSYS.2010.5442381

Cite this

Gong, Z., Nie, F., Zhang, T., Pathak, P., Wang, Z., Cui, K., Zhao, H., Wong, S., & Que, L. (2010). High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 1015-1018). Article 5442381 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442381

High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices. / Gong, Zhongcheng; Nie, Fang; Zhang, Tianhua et al.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 1015-1018 5442381 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Gong, Z, Nie, F, Zhang, T, Pathak, P, Wang, Z, Cui, K, Zhao, H , Wong, S & Que, L 2010, High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442381, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 1015-1018, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 1/24/10. https://doi.org/10.1109/MEMSYS.2010.5442381

Gong Z, Nie F, Zhang T, Pathak P, Wang Z, Cui K et al. High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 1015-1018. 5442381. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2010.5442381

Gong, Zhongcheng ; Nie, Fang ; Zhang, Tianhua et al. / High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices. MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. pp. 1015-1018 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "This paper reports the analysis of drug effects on single breast cancer cells using the droplet-microfluidic devices for the first time. Hundreds of droplets can be generated in less than 5 minutes. Each droplet serves as a nanoliter-volume titer plate. About 50% of the droplets contain a human breast cancer cell MDA-MB-231 and Cytochalasin D drug solution. The drug effects on each cell are monitored by analyzing the fluorescence image of each cell. Clear change of the cell shape and size has been observed after incubation, suggesting this approach is a potential viable technical platform for drug effect analysis and for high throughput drug screen and discovery.",

author = "Zhongcheng Gong and Fang Nie and Tianhua Zhang and Pushparaj Pathak and Zhiyong Wang and Kemi Cui and Hong Zhao and Stephen Wong and Long Que",

note = "Copyright: Copyright 2010 Elsevier B.V., All rights reserved.; 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

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AB - This paper reports the analysis of drug effects on single breast cancer cells using the droplet-microfluidic devices for the first time. Hundreds of droplets can be generated in less than 5 minutes. Each droplet serves as a nanoliter-volume titer plate. About 50% of the droplets contain a human breast cancer cell MDA-MB-231 and Cytochalasin D drug solution. The drug effects on each cell are monitored by analyzing the fluorescence image of each cell. Clear change of the cell shape and size has been observed after incubation, suggesting this approach is a potential viable technical platform for drug effect analysis and for high throughput drug screen and discovery.

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High throughput analysis of drug effects on single breast cancer cells using droplet-microfluidic devices

Abstract

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