HIV detection by in situ hybridization based on confocal reflected light microscopy

L. C. Smith; Z. Jericevic; R. Cuellar; S. Paddock; Dorothy E. Lewis

HIV detection by in situ hybridization based on confocal reflected light microscopy

L. C. Smith, Z. Jericevic, R. Cuellar, S. Paddock, Dorothy E. Lewis

Research output: Contribution to journal › Conference article › peer-review

Abstract

Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4⁺ cells (1 in 10⁴-10⁵) can be detected in the peripheral blood, even though there is dramatic depletion (often > 90%) of CD4⁺ cells as the disease progresses. A sensitive, ³⁵S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

Original language	English (US)
Pages (from-to)	224-232
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1428
State	Published - Jan 1 1991
Event	Proceedings of Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences - Los Angeles, CA, USA Duration: Jan 23 1991 → Jan 24 1991

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Cite this

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title = "HIV detection by in situ hybridization based on confocal reflected light microscopy",

abstract = "Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often > 90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.",

author = "Smith, {L. C.} and Z. Jericevic and R. Cuellar and S. Paddock and Lewis, {Dorothy E.}",

year = "1991",

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language = "English (US)",

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pages = "224--232",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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note = "Proceedings of Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences ; Conference date: 23-01-1991 Through 24-01-1991",

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TY - JOUR

T1 - HIV detection by in situ hybridization based on confocal reflected light microscopy

AU - Smith, L. C.

AU - Jericevic, Z.

AU - Cuellar, R.

AU - Paddock, S.

AU - Lewis, Dorothy E.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often > 90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

AB - Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often > 90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.

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AN - SCOPUS:0025791098

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VL - 1428

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JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Proceedings of Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences

Y2 - 23 January 1991 through 24 January 1991

ER -

HIV detection by in situ hybridization based on confocal reflected light microscopy

Abstract

ASJC Scopus subject areas

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