TY - CHAP
T1 - iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury
AU - Flinn, Hannah
AU - Cruz-Pineda, Leonardo
AU - Montier, Laura
AU - Horner, Philip J.
AU - Villapol, Sonia
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896–910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating three-dimensional (3D) imaging and quantification of cellular points and tissue structures. Once cleared, transparent tissue samples allow for rapid imaging with no mechanical sectioning. This imaging technology enables us to visualize brain tissue in situ and quantify the morphology and extent of glial cell branches or neuronal processes extending from the epicenter of a traumatic brain injury (TBI). In this way, we can more accurately assess and quantify the damaging consequences of TBI not only in the impact region but also in the extended pericontusional regions.
AB - Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896–910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating three-dimensional (3D) imaging and quantification of cellular points and tissue structures. Once cleared, transparent tissue samples allow for rapid imaging with no mechanical sectioning. This imaging technology enables us to visualize brain tissue in situ and quantify the morphology and extent of glial cell branches or neuronal processes extending from the epicenter of a traumatic brain injury (TBI). In this way, we can more accurately assess and quantify the damaging consequences of TBI not only in the impact region but also in the extended pericontusional regions.
KW - 3D imaging
KW - Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO)
KW - Light sheet microscopy
KW - Tissue clearing
KW - Whole-brain clearing
UR - http://www.scopus.com/inward/record.url?scp=85186372654&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85186372654&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-3662-6_39
DO - 10.1007/978-1-0716-3662-6_39
M3 - Chapter
C2 - 38427263
AN - SCOPUS:85186372654
T3 - Methods in Molecular Biology
SP - 589
EP - 597
BT - Methods in Molecular Biology
PB - Humana Press
ER -