TY - JOUR
T1 - Immunochemical studies of the intramolecular heterogeneity of the carcinoembryonic antigen (CEA) of the human digestive system
AU - Gold, Joshua Michael
AU - Banjo, C.
AU - Freedman, S. O.
AU - Gold, P.
PY - 1973/12/1
Y1 - 1973/12/1
N2 - Previous studies have shown that in addition to the tumor specific site on the carcinoembryonic antigen (CEA), this molecule also contains a blood group A like grouping. To study the A like site on the CEA molecule, a radioimmunoassay was devised in which anti A bodies were coupled to either Sepharose or Sephadex beads. The following observations were made. The monosaccharide, N acetyl D galactosamine was capable of inhibiting the interaction between 125I CEA and a preparation of anti A antibodies. A glycopeptide (GP 1), containing the tumor specific antigenic site of the CEA, which was obtained by the enzymatic degradation of the CEA molecule was capable of binding to anti A antibodies. The ratio, by weight, of GP 1 to N acetyl D galactosamine required to achieve equivalent binding was 10-3 to 10-4 to 1. GP 1 has a molecular weight of about 4000 daltons, and although it contains N acetyl D glucosamine, D mannose, D galactose, and L fucose, the glycopeptide is apparently devoid of N acetyl D galactosamine.
AB - Previous studies have shown that in addition to the tumor specific site on the carcinoembryonic antigen (CEA), this molecule also contains a blood group A like grouping. To study the A like site on the CEA molecule, a radioimmunoassay was devised in which anti A bodies were coupled to either Sepharose or Sephadex beads. The following observations were made. The monosaccharide, N acetyl D galactosamine was capable of inhibiting the interaction between 125I CEA and a preparation of anti A antibodies. A glycopeptide (GP 1), containing the tumor specific antigenic site of the CEA, which was obtained by the enzymatic degradation of the CEA molecule was capable of binding to anti A antibodies. The ratio, by weight, of GP 1 to N acetyl D galactosamine required to achieve equivalent binding was 10-3 to 10-4 to 1. GP 1 has a molecular weight of about 4000 daltons, and although it contains N acetyl D glucosamine, D mannose, D galactose, and L fucose, the glycopeptide is apparently devoid of N acetyl D galactosamine.
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M3 - Article
C2 - 4127301
AN - SCOPUS:0015712182
SN - 0022-1767
VL - 111
SP - 1872
EP - 1879
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -