Impact of administered bifidobacterium on murine host fatty acid composition

Rebecca Wall; R. Paul Ross; Fergus Shanahan; Liam O'Mahony; Barry Kiely; Eamonn Quigley; Timothy G. Dinan; Gerald Fitzgerald; Catherine Stanton

doi:10.1007/s11745-010-3410-7

Impact of administered bifidobacterium on murine host fatty acid composition

Rebecca Wall, R. Paul Ross, Fergus Shanahan, Liam O'Mahony, Barry Kiely, Eamonn Quigley, Timothy G. Dinan, Gerald Fitzgerald, Catherine Stanton

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, α-linolenic acid, on host fatty acid composition. α-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 10⁹ microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of α-linolenic acid resulted in higher (P < 0.05) α-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive α-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with α-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with α-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.

Original language	English (US)
Pages (from-to)	429-436
Number of pages	8
Journal	Lipids
Volume	45
Issue number	5
DOIs	https://doi.org/10.1007/s11745-010-3410-7
State	Published - May 2010

Keywords

Bifidobacteria
Docosahexaenoic acid
Eicosapentaenoic acid
Microbiota
Omega-3 fatty acids
Probiotics

ASJC Scopus subject areas

Biochemistry
Cell Biology
Organic Chemistry

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10.1007/s11745-010-3410-7

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title = "Impact of administered bifidobacterium on murine host fatty acid composition",

abstract = "Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, α-linolenic acid, on host fatty acid composition. α-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 109 microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of α-linolenic acid resulted in higher (P < 0.05) α-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive α-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with α-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with α-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.",

keywords = "Bifidobacteria, Docosahexaenoic acid, Eicosapentaenoic acid, Microbiota, Omega-3 fatty acids, Probiotics",

author = "Rebecca Wall and Ross, {R. Paul} and Fergus Shanahan and Liam O'Mahony and Barry Kiely and Eamonn Quigley and Dinan, {Timothy G.} and Gerald Fitzgerald and Catherine Stanton",

note = "Funding Information: Acknowledgments The authors acknowledge the technical assistance of Seamus Aherne for fatty acid analysis, Frances O{\textquoteright}Brien and Grainne Hurley for assistance with the murine trial. The authors are supported, in part, by Science Foundation Ireland (SFI), the Irish Ministry for Food and Agriculture, the Higher Education Authority and the Health Research Board of Ireland and the Irish Government under the National Development Plan 2000–2006.",

year = "2010",

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doi = "10.1007/s11745-010-3410-7",

language = "English (US)",

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AU - Wall, Rebecca

AU - Ross, R. Paul

AU - Shanahan, Fergus

AU - O'Mahony, Liam

AU - Kiely, Barry

AU - Quigley, Eamonn

AU - Dinan, Timothy G.

AU - Fitzgerald, Gerald

AU - Stanton, Catherine

N1 - Funding Information: Acknowledgments The authors acknowledge the technical assistance of Seamus Aherne for fatty acid analysis, Frances O’Brien and Grainne Hurley for assistance with the murine trial. The authors are supported, in part, by Science Foundation Ireland (SFI), the Irish Ministry for Food and Agriculture, the Higher Education Authority and the Health Research Board of Ireland and the Irish Government under the National Development Plan 2000–2006.

PY - 2010/5

Y1 - 2010/5

N2 - Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, α-linolenic acid, on host fatty acid composition. α-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 109 microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of α-linolenic acid resulted in higher (P < 0.05) α-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive α-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with α-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with α-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.

AB - Recently, we reported that administration of Bifidobacteria resulted in increased concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in murine adipose tissue [1]. The objective of this study was to assess the impact of co-administration of Bifidobacterium breve NCIMB 702258 and the substrate for EPA, α-linolenic acid, on host fatty acid composition. α-Linolenic acid-supplemented diets (1%, wt/wt) were fed to mice (n = 8), with or without B. breve NCIMB 702258 (daily dose of 109 microorganisms) for 8 weeks. Two further groups received either supplement of B. breve alone or unsupplemented diet. Tissue fatty acid composition was assessed by gas liquid chromatography. Dietary supplementation of α-linolenic acid resulted in higher (P < 0.05) α-linolenic acid and EPA concentrations in liver and adipose tissue and lower (P < 0.05) arachidonic acid in liver, adipose tissue and brain compared with mice that did not receive α-linolenic acid. Supplementation with B. breve NCIMB 702258 in combination with α-linolenic acid resulted in elevated (P < 0.05) liver EPA concentrations compared with α-linolenic acid supplementation alone. Furthermore, the former group had higher (P < 0.05) DHA in brain compared with the latter group. These results suggest a role for interactions between fatty acids and commensals in the gastrointestinal tract. This interaction between administered microbes and fatty acids could result in a highly effective nutritional approach to the therapy of a variety of inflammatory and neurodegenerative conditions.

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KW - Microbiota

KW - Omega-3 fatty acids

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Impact of administered bifidobacterium on murine host fatty acid composition

Abstract

Keywords

ASJC Scopus subject areas

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