In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb

Md Harun Al Rashid; Sayani Majumder; Vivekananda Mandal; Subhash C. Mandal; Rajarajan Amirthalingam Thandavarayan

doi:10.1016/j.jtcme.2017.11.003

In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb

Md Harun Al Rashid, Sayani Majumder, Vivekananda Mandal, Subhash C. Mandal, Rajarajan Amirthalingam Thandavarayan

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Diospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitative phytochemicals analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of D. melanoxylon were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from Diospyros melanoxylon leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The in-vitro antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant (P < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of D. Melanoxylon leaf extract due to presence of bioactive compounds.

Original language	English (US)
Pages (from-to)	106-118
Number of pages	13
Journal	Journal of Traditional and Complementary Medicine
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.jtcme.2017.11.003
State	Published - Apr 2019

Keywords

Antidiabetic
D. melanoxylon
Extraction techniques
HPLC
Polyphenolics

ASJC Scopus subject areas

Complementary and alternative medicine

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10.1016/j.jtcme.2017.11.003

Cite this

Harun Al Rashid, M., Majumder, S., Mandal, V., Mandal, S. C., & Amirthalingam Thandavarayan, R. (2019). In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. Journal of Traditional and Complementary Medicine, 9(2), 106-118. https://doi.org/10.1016/j.jtcme.2017.11.003

In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb. / Harun Al Rashid, Md; Majumder, Sayani; Mandal, Vivekananda et al.
In: Journal of Traditional and Complementary Medicine, Vol. 9, No. 2, 04.2019, p. 106-118.

Research output: Contribution to journal › Article › peer-review

Harun Al Rashid, M, Majumder, S, Mandal, V, Mandal, SC & Amirthalingam Thandavarayan, R 2019, 'In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb', Journal of Traditional and Complementary Medicine, vol. 9, no. 2, pp. 106-118. https://doi.org/10.1016/j.jtcme.2017.11.003

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title = "In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb",

abstract = "Diospyros melanoxylon Roxb. (D. melanoxylon) belongs to the family Ebenaceae and its leaves are very well known for making beedi throughout the World. The current study estimated the comparative extraction technique and its in-vitro antidiabetic prospective of the leaves of D. melanoxylon. Qualitative phytochemicals analysis of the samples from D. melanoxylon was carried out for the detection of secondary metabolites. Total phenolics, flavonoids, triterpenoids and tannins content of D. melanoxylon were estimated using colorimetric assay. Microwave-assisted extraction (MAE) technique with a low carbon output was observed for the speedy extraction of bioactive compounds obtained from Diospyros melanoxylon leaf extract. MAE produced a maximum yield of bioactive compounds which was found to be more efficient than ultrasound, soxhlet and maceration extraction. Qualitative HPLC analysis was performed for bioactive compounds. The in-vitro antidiabetic assay was performed using α-amylase and α-glucosidase inhibitory activity. In conclusion, the fractions exhibited the concentration-dependent inhibitory effect with significant (P < 0.0001) result. So the above performance might be accountable for the antidiabetic activity of D. Melanoxylon leaf extract due to presence of bioactive compounds.",

keywords = "Antidiabetic, D. melanoxylon, Extraction techniques, HPLC, Polyphenolics",

author = "{Harun Al Rashid}, Md and Sayani Majumder and Vivekananda Mandal and Mandal, {Subhash C.} and {Amirthalingam Thandavarayan}, Rajarajan",

note = "Funding Information: We are grateful to “Innovation in Science Pursuit for Inspire Research” (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Funding Information: We are grateful to ?Innovation in Science Pursuit for Inspire Research? (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Funding Information: We are grateful to “Innovation in Science Pursuit for Inspire Research” (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613 ) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Publisher Copyright: {\textcopyright} 2017 Center for Food and Biomolecules, National Taiwan University",

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AU - Mandal, Subhash C.

AU - Amirthalingam Thandavarayan, Rajarajan

N1 - Funding Information: We are grateful to “Innovation in Science Pursuit for Inspire Research” (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Funding Information: We are grateful to ?Innovation in Science Pursuit for Inspire Research? (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Funding Information: We are grateful to “Innovation in Science Pursuit for Inspire Research” (INSPIRE) implemented by the Department of Science and Technology (No. DST/ INSPIRE Fellowship/2013/613 ) for financial support. The authors are also thankful to the authority of Jadavpur University and Bose Institute, Kolkata for affording necessary facilities. Publisher Copyright: © 2017 Center for Food and Biomolecules, National Taiwan University

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In search of suitable extraction technique for large scale commercial production of bioactive fraction for the treatment of diabetes: The case Diospyros melanoxylon Roxb

Abstract

Keywords

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