Abstract
Metastasis is responsible for 90% of human cancer mortality, yet it remains a challenge to model human cancer metastasis in vivo. Here we describe mouse models of high-grade serous ovarian cancer, also known as high-grade serous carcinoma (HGSC), the most common and deadliest human ovarian cancer type. Mice genetically engineered to harbor Dicer1 and Pten inactivation and mutant p53 robustly replicate the peritoneal metastases of human HGSC with complete penetrance. Arising from the fallopian tube, tumors spread to the ovary and metastasize throughout the pelvic and peritoneal cavities, invariably inducing hemorrhagic ascites. Widespread and abundant peritoneal metastases ultimately cause mouse deaths (100%). Besides the phenotypic and histopathological similarities, mouse HGSCs also display marked chromosomal instability, impaired DNA repair, and chemosensitivity. Faithfully recapitulating the clinical metastases as well as molecular and genomic features of human HGSC, this murine model will be valuable for elucidating the mechanisms underlying the development and progression of metastatic ovarian cancer and also for evaluating potential therapies.
Original language | English (US) |
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Article number | e1008808 |
Pages (from-to) | e1008808 |
Journal | PLoS Genetics |
Volume | 16 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2020 |
Keywords
- Animals
- Antineoplastic Agents/pharmacology
- Cell Line, Tumor
- Chromosomal Instability
- Cystadenocarcinoma, Serous/drug therapy
- DEAD-box RNA Helicases/genetics
- DNA Repair
- Disease Models, Animal
- Drug Resistance, Neoplasm/genetics
- Drug Screening Assays, Antitumor/methods
- Feasibility Studies
- Female
- Humans
- Mice
- Mice, Knockout
- Mutation
- Neoplasm Grading
- Neoplasm Metastasis/genetics
- Ovarian Neoplasms/drug therapy
- PTEN Phosphohydrolase/genetics
- Peritoneal Neoplasms/drug therapy
- Primary Cell Culture
- Ribonuclease III/genetics
- Tumor Suppressor Protein p53/genetics
ASJC Scopus subject areas
- Genetics(clinical)
- Genetics
- Ecology, Evolution, Behavior and Systematics
- Molecular Biology
- Cancer Research