TY - JOUR
T1 - Induction of cellular DNA synthesis by polyoma virus. II. Increase in the rate of enzyme synthesis after infection with polyoma virus in mouse kidney cells
AU - Hartwell, L. H.
AU - Vogt, M.
AU - Dulbecco, R.
N1 - Funding Information:
1 Aided by Grant CA-07592-01 from The National Cancer Institute, National Institutes of Health. 2 Supported by a Fellowship from the Air Force Office of Scientific Research and the National Academy of Sciences. 8 Present address: Department of Molecular and Cell Biology, University of California, Irvine. 4 Abbreviations, DNA, deoxyribonucleic acid; RNA, ribonucleic acid; dAMP, deoxyadenylic acid; dCMP, deoxycytidylic acid; dCTP, deoxycytidine 5’-triphosphate; dUMP, deoxyuridylic
PY - 1965/11
Y1 - 1965/11
N2 - An induction of cellular DNA synthesis and an increase in the activity of three enzymes involved in DNA synthesis, dCMP deaminase, TdR Kinase, and DNA polymerase, following the infection of mouse kidney cells with polyoma virus was described previously (Dulbecco et al., 1965). Experiments described in this paper elucidate the mechanism of the increase in enzymatic activities of dCMP deaminase and TdR kinase. Three alternative mechanisms are considered: (1) an increase in the rate of enzyme synthesis, (2) a stabilization of labile enzyme molecules, and (3) an activation of enzyme activity. Experimental evidence is presented which rules out the latter two possibilities. We conclude therefore that the rate of synthesis of dCMP deaminase and TdR kinase is increased as a result of polyoma infection.
AB - An induction of cellular DNA synthesis and an increase in the activity of three enzymes involved in DNA synthesis, dCMP deaminase, TdR Kinase, and DNA polymerase, following the infection of mouse kidney cells with polyoma virus was described previously (Dulbecco et al., 1965). Experiments described in this paper elucidate the mechanism of the increase in enzymatic activities of dCMP deaminase and TdR kinase. Three alternative mechanisms are considered: (1) an increase in the rate of enzyme synthesis, (2) a stabilization of labile enzyme molecules, and (3) an activation of enzyme activity. Experimental evidence is presented which rules out the latter two possibilities. We conclude therefore that the rate of synthesis of dCMP deaminase and TdR kinase is increased as a result of polyoma infection.
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U2 - 10.1016/0042-6822(65)90105-4
DO - 10.1016/0042-6822(65)90105-4
M3 - Article
C2 - 4285100
AN - SCOPUS:0013812259
SN - 0042-6822
VL - 27
SP - 262
EP - 272
JO - Virology
JF - Virology
IS - 3
ER -