TY - JOUR
T1 - Inhalable Microparticles Embedding Biocompatible Magnetic Iron-Doped Hydroxyapatite Nanoparticles
AU - Quarta, Eride
AU - Chiappi, Michele
AU - Adamiano, Alessio
AU - Tampieri, Anna
AU - Wang, Weijie
AU - Tetley, Teresa D.
AU - Buttini, Francesca
AU - Sonvico, Fabio
AU - Catalucci, Daniele
AU - Colombo, Paolo
AU - Iafisco, Michele
AU - Degli Esposti, Lorenzo
N1 - Funding Information:
This research was funded by the European Union’s Horizon 2020 research and innovation program under project CUPIDO, grant agreement [No. 720834].
Funding Information:
The Facility for Imaging by Light Microscopy (FILM) at Imperial College London is part-supported by funding from the Wellcome Trust (grant 104931/Z/14/Z) and BBSRC (grant BB/L015129/1).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/3/28
Y1 - 2023/3/28
N2 - Recently, there has been increasing interest in developing biocompatible inhalable nanoparticle formulations, as they have enormous potential for treating and diagnosing lung disease. In this respect, here, we have studied superparamagnetic iron-doped calcium phosphate (in the form of hydroxyapatite) nanoparticles (FeCaP NPs) which were previously proved to be excellent materials for magnetic resonance imaging, drug delivery and hyperthermia-related applications. We have established that FeCaP NPs are not cytotoxic towards human lung alveolar epithelial type 1 (AT1) cells even at high doses, thus proving their safety for inhalation administration. Then, D-mannitol spray-dried microparticles embedding FeCaP NPs have been formulated, obtaining respirable dry powders. These microparticles were designed to achieve the best aerodynamic particle size distribution which is a critical condition for successful inhalation and deposition. The nanoparticle-in-microparticle approach resulted in the protection of FeCaP NPs, allowing their release upon microparticle dissolution, with dimensions and surface charge close to the original values. This work demonstrates the use of spray drying to provide an inhalable dry powder platform for the lung delivery of safe FeCaP NPs for magnetically driven applications.
AB - Recently, there has been increasing interest in developing biocompatible inhalable nanoparticle formulations, as they have enormous potential for treating and diagnosing lung disease. In this respect, here, we have studied superparamagnetic iron-doped calcium phosphate (in the form of hydroxyapatite) nanoparticles (FeCaP NPs) which were previously proved to be excellent materials for magnetic resonance imaging, drug delivery and hyperthermia-related applications. We have established that FeCaP NPs are not cytotoxic towards human lung alveolar epithelial type 1 (AT1) cells even at high doses, thus proving their safety for inhalation administration. Then, D-mannitol spray-dried microparticles embedding FeCaP NPs have been formulated, obtaining respirable dry powders. These microparticles were designed to achieve the best aerodynamic particle size distribution which is a critical condition for successful inhalation and deposition. The nanoparticle-in-microparticle approach resulted in the protection of FeCaP NPs, allowing their release upon microparticle dissolution, with dimensions and surface charge close to the original values. This work demonstrates the use of spray drying to provide an inhalable dry powder platform for the lung delivery of safe FeCaP NPs for magnetically driven applications.
KW - Trojan microparticles
KW - hydroxyapatite
KW - inhalable dry powder
KW - microparticles embedding nanoparticles
KW - nanomedicine
KW - pulmonary disease
KW - superparamagnetic nanoparticles
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U2 - 10.3390/jfb14040189
DO - 10.3390/jfb14040189
M3 - Article
C2 - 37103279
AN - SCOPUS:85153860790
SN - 2079-4983
VL - 14
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
IS - 4
M1 - 189
ER -