TY - JOUR
T1 - Isolation of a helical, lipid-binding fragment from the human plasma high density lipoprotein, apolp-GLN-I
AU - Jackson, R. L.
AU - Baker, H. N.
AU - David, J. S.K.
AU - Gotto, Antonio
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1972/12/18
Y1 - 1972/12/18
N2 - The major apolipoprotein of human plasma high density lipoproteins (HDL), apoLP-Gln-I, has previously been shown to have a high content of α-helical conformation and a molecular weight of approximately 28,000. Our results show that apoLP-Gln-I contains three methionine residues. We have treated apoLP-Gln-I with cyanogen bromide (CNBr) and have isolated the four CNBr fragments by chromatography on BioGel P-30. One of the fragments, CNBr-I has 92 amino acid residues, contains no homoserine or methionine and corresponds to the carboxyl-terminal peptide. This fragment is of particular interest since it was shown to retain a high content of α-helix, while the other fragments were mainly disordered, and to possess the ability to bind phosphatidyl choline.
AB - The major apolipoprotein of human plasma high density lipoproteins (HDL), apoLP-Gln-I, has previously been shown to have a high content of α-helical conformation and a molecular weight of approximately 28,000. Our results show that apoLP-Gln-I contains three methionine residues. We have treated apoLP-Gln-I with cyanogen bromide (CNBr) and have isolated the four CNBr fragments by chromatography on BioGel P-30. One of the fragments, CNBr-I has 92 amino acid residues, contains no homoserine or methionine and corresponds to the carboxyl-terminal peptide. This fragment is of particular interest since it was shown to retain a high content of α-helix, while the other fragments were mainly disordered, and to possess the ability to bind phosphatidyl choline.
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U2 - 10.1016/0006-291X(72)90501-3
DO - 10.1016/0006-291X(72)90501-3
M3 - Article
C2 - 4344811
AN - SCOPUS:0015515440
SN - 0006-291X
VL - 49
SP - 1444
EP - 1451
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 6
ER -