Abstract
Cell-based therapies harness functional cells or tissues to mediate healing and treat disease. Assessment of cellular therapeutics requires methods that are non-destructive to ensure therapies remain viable and uncontaminated for use in patients. Optical imaging of endogenous collagen, by second-harmonic generation, and the metabolic coenzymes NADH and FAD, by autofluorescence microscopy, provides tissue structure and cellular information. Here, we review applications of label-free nonlinear optical imaging of cellular metabolism and collagen second-harmonic generation for assessing cell-based therapies. Additionally, we discuss the potential of label-free imaging for quality control of cell-based therapies, as well as the current limitations and potential future directions of label-free imaging technologies.
Original language | English (US) |
---|---|
Article number | 100433 |
Journal | Current Opinion in Biomedical Engineering |
Volume | 25 |
DOIs | |
State | Published - Mar 2023 |
Keywords
- Collagen
- Cytotherapy
- Fluorescence
- Metabolism
- Microscopy
- Regenerative engineering
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomaterials
- Biomedical Engineering