TY - JOUR
T1 - Live cell imaging fails to support viral-protein-mediated intercellular trafficking
AU - Wu, Fuqing
AU - Long, Jing
AU - Wang, Shuai
AU - Xing, Junji
AU - Li, Meili
AU - Zheng, Chunfu
N1 - Funding Information:
We are grateful to Dr. Rozanne M. Sandri-Goldin for critical review of the manuscript. We thank Dr. Liu Zhengfei for the generous gift of the BHV-1 strain, and NIH for the plasmid pNL4-3. This work was supported by grants from the Major State Basic Research Development Program of China (973 Program, 2010CB530105 and 2011CB504802) and National Natural Science Foundation of China (30870120, 30900059 and 81000736).
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - The herpes simplex virus type I protein VP22 has been reported to have the property of intercellular trafficking. However, there is little direct evidence to demonstrate that VP22 can shuttle freely between living cells. Here, we employ a novel and simple assay using live cell fluorescence microscopy to investigate the intercellular transport property. Our results demonstrated that VP22, bovine herpesvirus-1 VP22, HSV-1 US11 and HIV Tat could not shuttle into neighboring cells via direct contact.
AB - The herpes simplex virus type I protein VP22 has been reported to have the property of intercellular trafficking. However, there is little direct evidence to demonstrate that VP22 can shuttle freely between living cells. Here, we employ a novel and simple assay using live cell fluorescence microscopy to investigate the intercellular transport property. Our results demonstrated that VP22, bovine herpesvirus-1 VP22, HSV-1 US11 and HIV Tat could not shuttle into neighboring cells via direct contact.
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U2 - 10.1007/s00705-012-1308-9
DO - 10.1007/s00705-012-1308-9
M3 - Article
C2 - 22527865
AN - SCOPUS:84863110741
SN - 0304-8608
VL - 157
SP - 1383
EP - 1386
JO - Archives of Virology
JF - Archives of Virology
IS - 7
ER -