@article{ab3ecfa3956d48f18eaa5e1fc7ccfbd9,
title = "Long noncoding RNAs are substrates for cytoplasmic capping enzyme",
abstract = "Cytoplasmic capping returns a cap to specific mRNAs, thus protecting uncapped RNAs from decay. Prior to the identification of cytoplasmic capping, uncapped mRNAs were thought to be degraded. Here, we test whether long noncoding RNAs (lncRNAs) are substrates of the cytoplasmic capping enzyme (cCE). The subcellular localisation of 14 lncRNAs associated with sarcomas were examined in U2OS osteosarcoma cells. We used 5′ rapid amplification of cDNA ends (RACE) to assay uncapped forms of these lncRNAs. Inhibiting cytoplasmic capping elevated uncapped forms of selected lncRNAs indicating a plausible role of cCE in targeting them. Analysis of published cap analysis of gene expression (CAGE) data shows increased prevalence of certain 5{\textquoteright}-RACE cloned sequences, suggesting that these uncapped lncRNAs are targets of cytoplasmic capping.",
keywords = "5′-RACE, CAGE, cytoplasmic capping, lncRNA, uncapped RNA ends, RNA, Long Noncoding/genetics, RNA Caps/genetics, Cytoplasm/metabolism, Cytosol/metabolism, RNA, Messenger/genetics",
author = "Avik Mukherjee and Safirul Islam and Kieser, {Rachel E.} and Kiss, {Daniel L.} and Chandrama Mukherjee",
note = "Funding Information: This work was supported by an extramural grant CRG/2019/006427 from the Department of Science and Technology, Government of India to Dr Chandrama Mukherjee. The authors thank SERB‐CRG for providing fellowship to Mr. Avik Mukherjee. The authors would also like to thank UGC, Government of India for a UGC‐SRF fellowship to Mr. Safirul Islam and the Department of Biotechnology, India for a Ramlingaswami Re‐entry fellowship to Dr Chandrama Mukherjee. The authors would like to thank Presidency University for the necessary infrastructural support, Dr Piyali Mukherjee, Presidency University for providing Lamin antibody, Dr Shubhra Majumder, Presidency University for providing HA antibody and Dr Abhik Saha, Presidency University for providing access to Licor imager. The Kiss Lab's contributions are supported by a grant from the National Institutes of Health (R35GM137819, to DLK) and Dr Rachel Kieser is supported by a postdoctoral supplement (R35GM137819‐03S3). Funding Information: This work was supported by an extramural grant CRG/2019/006427 from the Department of Science and Technology, Government of India to Dr Chandrama Mukherjee. The authors thank SERB-CRG for providing fellowship to Mr. Avik Mukherjee. The authors would also like to thank UGC, Government of India for a UGC-SRF fellowship to Mr. Safirul Islam and the Department of Biotechnology, India for a Ramlingaswami Re-entry fellowship to Dr Chandrama Mukherjee. The authors would like to thank Presidency University for the necessary infrastructural support, Dr Piyali Mukherjee, Presidency University for providing Lamin antibody, Dr Shubhra Majumder, Presidency University for providing HA antibody and Dr Abhik Saha, Presidency University for providing access to Licor imager. The Kiss Lab's contributions are supported by a grant from the National Institutes of Health (R35GM137819, to DLK) and Dr Rachel Kieser is supported by a postdoctoral supplement (R35GM137819-03S3). Publisher Copyright: {\textcopyright} 2023 Federation of European Biochemical Societies.",
year = "2023",
month = apr,
doi = "10.1002/1873-3468.14603",
language = "English (US)",
volume = "597",
pages = "947--961",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "7",
}