Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry

B. J. Smith; D. A. Herold; R. M. Ross; F. G. Marquis; R. L. Bertholf; C. R. Ayers; M. R. Wills; J. Savory

Measurement of plasma prostaglandin E₂ using capillary gas chromatography negative ion chemical ionization mass spectrometry

B. J. Smith, D. A. Herold, R. M. Ross, F. G. Marquis, R. L. Bertholf, C. R. Ayers, M. R. Wills, J. Savory

Research output: Contribution to journal › Article › peer-review

Abstract

A stable isotope dilution assay for the measurement of plasma prostaglandin E₂ (PGE₂) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE₂ was extracted from plasma using C₁₈ and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethylsilyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE₂ determined in 8 healthy volunteers was 2.8 ± 2.0 pg/ml.

Original language	English (US)
Pages (from-to)	73-86
Number of pages	14
Journal	Research Communications in Chemical Pathology and Pharmacology
Volume	40
Issue number	1
State	Published - 1983

ASJC Scopus subject areas

Pathology and Forensic Medicine
Toxicology
Pharmacology
Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry",

abstract = "A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethylsilyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 ± 2.0 pg/ml.",

author = "Smith, {B. J.} and Herold, {D. A.} and Ross, {R. M.} and Marquis, {F. G.} and Bertholf, {R. L.} and Ayers, {C. R.} and Wills, {M. R.} and J. Savory",

year = "1983",

language = "English (US)",

volume = "40",

pages = "73--86",

journal = "Research Communications in Chemical Pathology and Pharmacology",

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T1 - Measurement of plasma prostaglandin E2 using capillary gas chromatography negative ion chemical ionization mass spectrometry

AU - Smith, B. J.

AU - Herold, D. A.

AU - Ross, R. M.

AU - Marquis, F. G.

AU - Bertholf, R. L.

AU - Ayers, C. R.

AU - Wills, M. R.

AU - Savory, J.

PY - 1983

Y1 - 1983

N2 - A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethylsilyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 ± 2.0 pg/ml.

AB - A stable isotope dilution assay for the measurement of plasma prostaglandin E2 (PGE2) employing capillary column gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS) is described. PGE2 was extracted from plasma using C18 and silica SEP-PAKS. Further purification and separation was accomplished by thin layer chromatography. The prostaglandin was analyzed as its pentafluorobenzyl ester-methoxime-trimethylsilyl ether, using fragment ions at m/e 524 (protium) and m/e 528 (deuterium) for quantitation. The mean plasma concentration of PGE2 determined in 8 healthy volunteers was 2.8 ± 2.0 pg/ml.

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SN - 0034-5164

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JO - Research Communications in Chemical Pathology and Pharmacology

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Measurement of plasma prostaglandin E₂ using capillary gas chromatography negative ion chemical ionization mass spectrometry

Abstract

ASJC Scopus subject areas

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