mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine

Xiaoyu Gao; Yao Li; Guangjun Nie; Xiao Zhao

doi:10.21769/BioProtoc.4774

mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine

Xiaoyu Gao, Yao Li, Guangjun Nie, Xiao Zhao

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.

Original language	English (US)
Article number	e4774
Pages (from-to)	e4774
Journal	Bio-protocol
Volume	13
Issue number	13
DOIs	https://doi.org/10.21769/BioProtoc.4774
State	Published - Jul 5 2023

Keywords

BoxC/D
Cancer immunotherapy
mRNA vaccines
Outer membrane vesicles
Rapid display
RNA binding protein

ASJC Scopus subject areas

Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
Plant Science

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10.21769/BioProtoc.4774

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title = "mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine",

abstract = "The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding. ",

keywords = "BoxC/D, Cancer immunotherapy, mRNA vaccines, Outer membrane vesicles, Rapid display, RNA binding protein",

author = "Xiaoyu Gao and Yao Li and Guangjun Nie and Xiao Zhao",

note = "Funding Information: This work was supported by grants from the National Key R&D Program of China (2022YFB3808100 and 2021YFA0909900, X.Z.), the CAS Project for Young Scientists in Basic Research (YSBR-010, X.Z.), the Beijing Natural Science Foundation (Z200020, X.Z.), the Beijing Nova Program (Z201100006820031, X.Z.) and the National Natural Science Foundation of China (32222045 and 32171384, X.Z.). This protocol was adapted from a previous study (Li et al., 2022). Publisher Copyright: Copyright: {\textcopyright} 2023 The Authors; exclusive licensee Bio-protocol LLC.",

year = "2023",

month = jul,

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doi = "10.21769/BioProtoc.4774",

language = "English (US)",

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AU - Gao, Xiaoyu

AU - Li, Yao

AU - Nie, Guangjun

AU - Zhao, Xiao

N1 - Funding Information: This work was supported by grants from the National Key R&D Program of China (2022YFB3808100 and 2021YFA0909900, X.Z.), the CAS Project for Young Scientists in Basic Research (YSBR-010, X.Z.), the Beijing Natural Science Foundation (Z200020, X.Z.), the Beijing Nova Program (Z201100006820031, X.Z.) and the National Natural Science Foundation of China (32222045 and 32171384, X.Z.). This protocol was adapted from a previous study (Li et al., 2022). Publisher Copyright: Copyright: © 2023 The Authors; exclusive licensee Bio-protocol LLC.

PY - 2023/7/5

Y1 - 2023/7/5

N2 - The rapid display and delivery method for customized tumor mRNA vaccines is limited. Herein, bacteria-derived outer membrane vesicles (OMVs) are employed as an mRNA delivery platform by surface engineering of an RNA-binding protein, L7Ae. OMV-L7Ae can rapidly adsorb boxC/D sequence-labeled mRNA antigens through L7Ae-boxC/D binding and deliver them into HEK-293T and dendritic cells. This platform provides an mRNA delivery technology distinct from lipid nanoparticles (LNPs) for personalized mRNA tumor vaccination and with a Plug-and-Display strategy suitable for rapid preparation of the personalized mRNA tumor vaccine against varied tumor antigens. Key features OMVs are employed as an mRNA delivery platform through L7Ae-boxC/D binding.

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KW - Rapid display

KW - RNA binding protein

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mRNA Delivery Platform Based on Bacterial Outer Membrane Vesicles for Tumor Vaccine

Abstract

Keywords

ASJC Scopus subject areas

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