Novel suction-based in vivo cutaneous DNA transfection platform

Emran O. Lallow; Nandita C. Jhumur; Ijaz Ahmed; Sagar B. Kudchodkar; Christine C. Roberts; Moonsup Jeong; Juliet M. Melnik; Sarah H. Park; Kar Muthumani; Jerry W. Shan; Jeffrey D. Zahn; David I. Shreiber; Jonathan P. Singer; Young K. Park; Joel N. Maslow; Hao Lin

doi:10.1126/sciadv.abj0611

Novel suction-based in vivo cutaneous DNA transfection platform

Emran O. Lallow, Nandita C. Jhumur, Ijaz Ahmed, Sagar B. Kudchodkar, Christine C. Roberts, Moonsup Jeong, Juliet M. Melnik, Sarah H. Park, Kar Muthumani, Jerry W. Shan, Jeffrey D. Zahn, David I. Shreiber, Jonathan P. Singer, Young K. Park, Joel N. Maslow, Hao Lin

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13 Scopus citations

Abstract

This work reports a suction-based cutaneous delivery method for in vivo DNA transfection. Following intradermal Mantoux injection of plasmid DNA in a rat model, a moderate negative pressure is applied to the injection site, a technique similar to Chinese báguàn and Middle Eastern hijama cupping therapies. Strong GFP expression was demonstrated with pEGFP-N1 plasmids where fluorescence was observed as early as 1 hour after dosing. Modeling indicates a strong correlation between focal strain/stress and expression patterns. The absence of visible and/or histological tissue injury contrasts with current in vivo transfection systems such as electroporation. Specific utility was demonstrated with a synthetic SARS-CoV-2 DNA vaccine, which generated host humoral immune response in rats with notable antibody production. This method enables an easy-to-use, cost-effective, and highly scalable platform for both laboratorial transfection needs and clinical applications for nucleic acid–based therapeutics and vaccines.

Original language	English (US)
Article number	eabj0611
Journal	Science Advances
Volume	7
Issue number	45
DOIs	https://doi.org/10.1126/sciadv.abj0611
State	Published - Nov 2021

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Lallow, E. O., Jhumur, N. C., Ahmed, I., Kudchodkar, S. B., Roberts, C. C., Jeong, M., Melnik, J. M., Park, S. H., Muthumani, K., Shan, J. W., Zahn, J. D., Shreiber, D. I., Singer, J. P., Park, Y. K., Maslow, J. N., & Lin, H. (2021). Novel suction-based in vivo cutaneous DNA transfection platform. Science Advances, 7(45), Article eabj0611. https://doi.org/10.1126/sciadv.abj0611

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title = "Novel suction-based in vivo cutaneous DNA transfection platform",

abstract = "This work reports a suction-based cutaneous delivery method for in vivo DNA transfection. Following intradermal Mantoux injection of plasmid DNA in a rat model, a moderate negative pressure is applied to the injection site, a technique similar to Chinese b{\'a}gu{\`a}n and Middle Eastern hijama cupping therapies. Strong GFP expression was demonstrated with pEGFP-N1 plasmids where fluorescence was observed as early as 1 hour after dosing. Modeling indicates a strong correlation between focal strain/stress and expression patterns. The absence of visible and/or histological tissue injury contrasts with current in vivo transfection systems such as electroporation. Specific utility was demonstrated with a synthetic SARS-CoV-2 DNA vaccine, which generated host humoral immune response in rats with notable antibody production. This method enables an easy-to-use, cost-effective, and highly scalable platform for both laboratorial transfection needs and clinical applications for nucleic acid–based therapeutics and vaccines.",

author = "Lallow, {Emran O.} and Jhumur, {Nandita C.} and Ijaz Ahmed and Kudchodkar, {Sagar B.} and Roberts, {Christine C.} and Moonsup Jeong and Melnik, {Juliet M.} and Park, {Sarah H.} and Kar Muthumani and Shan, {Jerry W.} and Zahn, {Jeffrey D.} and Shreiber, {David I.} and Singer, {Jonathan P.} and Park, {Young K.} and Maslow, {Joel N.} and Hao Lin",

note = "Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved;",

year = "2021",

month = nov,

doi = "10.1126/sciadv.abj0611",

language = "English (US)",

volume = "7",

journal = "Science Advances",

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AU - Lallow, Emran O.

AU - Jhumur, Nandita C.

AU - Ahmed, Ijaz

AU - Kudchodkar, Sagar B.

AU - Roberts, Christine C.

AU - Jeong, Moonsup

AU - Melnik, Juliet M.

AU - Park, Sarah H.

AU - Muthumani, Kar

AU - Shan, Jerry W.

AU - Zahn, Jeffrey D.

AU - Shreiber, David I.

AU - Singer, Jonathan P.

AU - Park, Young K.

AU - Maslow, Joel N.

AU - Lin, Hao

PY - 2021/11

Y1 - 2021/11

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AB - This work reports a suction-based cutaneous delivery method for in vivo DNA transfection. Following intradermal Mantoux injection of plasmid DNA in a rat model, a moderate negative pressure is applied to the injection site, a technique similar to Chinese báguàn and Middle Eastern hijama cupping therapies. Strong GFP expression was demonstrated with pEGFP-N1 plasmids where fluorescence was observed as early as 1 hour after dosing. Modeling indicates a strong correlation between focal strain/stress and expression patterns. The absence of visible and/or histological tissue injury contrasts with current in vivo transfection systems such as electroporation. Specific utility was demonstrated with a synthetic SARS-CoV-2 DNA vaccine, which generated host humoral immune response in rats with notable antibody production. This method enables an easy-to-use, cost-effective, and highly scalable platform for both laboratorial transfection needs and clinical applications for nucleic acid–based therapeutics and vaccines.

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Novel suction-based in vivo cutaneous DNA transfection platform

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