TY - GEN
T1 - Optical coherence tomography in quantifying the permeation of human plasma lipoproteins in vascular tissues
AU - Ghosn, M. G.
AU - Mashiatulla, M.
AU - Tuchin, V. V.
AU - Morrisett, J. D.
AU - Larin, K. V.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Atherosclerosis is the most common underlying cause of vascular disease, occurring in multiple arterial beds including the carotid, coronary, and femoral arteries. Atherosclerosis is an inflammatory process occurring in arterial tissue, involving the subintimal accumulation of low-density lipoproteins (LDL). Little is known about the rates at which these accumulations occur. Measurements of the permeability rate of LDL, and other lipoproteins such as high-density lipoprotein (HDL) and very low-density lipoprotein (VLDL), could help gain a better understanding of the mechanisms involved in the development of atherosclerotic lesions. The permeation of VLDL, LDL, HDL, and glucose was monitored and quantified in normal and diseased human carotid endarterectomy tissues at 20°C and 37°C using optical coherence tomography (OCT). The rates for LDL permeation through normal tissue at 20°C was (3.16 ± 0.37) ×10 -5 cm/sec and at 37°C was (4.77 ± 0.48) ×10 -5 cm/sec, significantly greater (p<0.05) than the rates for diseased tissue at these temperatures ((1.97 ± 0.34) ×10 -5 cm/sec and (2.01 ± 0.23) ×10 -5 cm/sec, respectively). The observed results support previous suggestions of an enhanced transport mechanism specific to LDL. This study effectively uses optical coherence tomography to measure the rates of permeation of vascular tissue by the range of naturally occurring lipoproteins.
AB - Atherosclerosis is the most common underlying cause of vascular disease, occurring in multiple arterial beds including the carotid, coronary, and femoral arteries. Atherosclerosis is an inflammatory process occurring in arterial tissue, involving the subintimal accumulation of low-density lipoproteins (LDL). Little is known about the rates at which these accumulations occur. Measurements of the permeability rate of LDL, and other lipoproteins such as high-density lipoprotein (HDL) and very low-density lipoprotein (VLDL), could help gain a better understanding of the mechanisms involved in the development of atherosclerotic lesions. The permeation of VLDL, LDL, HDL, and glucose was monitored and quantified in normal and diseased human carotid endarterectomy tissues at 20°C and 37°C using optical coherence tomography (OCT). The rates for LDL permeation through normal tissue at 20°C was (3.16 ± 0.37) ×10 -5 cm/sec and at 37°C was (4.77 ± 0.48) ×10 -5 cm/sec, significantly greater (p<0.05) than the rates for diseased tissue at these temperatures ((1.97 ± 0.34) ×10 -5 cm/sec and (2.01 ± 0.23) ×10 -5 cm/sec, respectively). The observed results support previous suggestions of an enhanced transport mechanism specific to LDL. This study effectively uses optical coherence tomography to measure the rates of permeation of vascular tissue by the range of naturally occurring lipoproteins.
KW - Atherosclerosis
KW - Carotid endarterectomy
KW - Lipoproteins
KW - Optical coherence tomography
KW - Permeability rate
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U2 - 10.1117/12.909297
DO - 10.1117/12.909297
M3 - Conference contribution
AN - SCOPUS:84860691632
SN - 9780819488657
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Dynamics and Fluctuations in Biomedical Photonics IX
T2 - Dynamics and Fluctuations in Biomedical Photonics IX
Y2 - 21 January 2012 through 23 January 2012
ER -