Optical metabolic imaging of live tissue cultures

Alex J. Walsh; Rebecca S. Cook; Carlos L. Arteag; Melissa C. Skala

doi:10.1117/12.2001863

Optical metabolic imaging of live tissue cultures

Alex J. Walsh, Rebecca S. Cook, Carlos L. Arteag, Melissa C. Skala

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Scopus citations

Abstract

The fluorescence properties, both intensity and fluorescence lifetime, of NADH and FAD, two coenzymes of metabolism, are sensitive, high resolution measures of cellular metabolism. However, often in vivo measurements of tissue are not feasible. In this study, we investigate the stability over time of two-photon auto-fluorescence imaging of NADH and FAD in live-cultured tissues. Our results demonstrate that cultured tissues remain viable for at least several days post excision. Furthermore, the optical redox ratio, NADH fluorescence lifetime, and FAD fluorescence lifetime do not significantly change in the cultured tissues over time. With these findings, we demonstrate the potential of sustained tissue culture techniques for optical metabolic imaging.

Original language	English (US)
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIII
DOIs	https://doi.org/10.1117/12.2001863
State	Published - 2013
Event	Multiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States Duration: Feb 3 2013 → Feb 5 2013

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	8588
ISSN (Print)	1605-7422

Other

Other	Multiphoton Microscopy in the Biomedical Sciences XIII
Country/Territory	United States
City	San Francisco, CA
Period	2/3/13 → 2/5/13

Keywords

Cancer
Cellular metabolism
Clinical translation
Multi-photon fluorescence

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2001863

Cite this

Walsh, AJ, Cook, RS, Arteag, CL & Skala, MC 2013, Optical metabolic imaging of live tissue cultures. in Multiphoton Microscopy in the Biomedical Sciences XIII., 858820, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, San Francisco, CA, United States, 2/3/13. https://doi.org/10.1117/12.2001863

@inproceedings{57e4df9375f14856bccdf887d21fd801,

title = "Optical metabolic imaging of live tissue cultures",

abstract = "The fluorescence properties, both intensity and fluorescence lifetime, of NADH and FAD, two coenzymes of metabolism, are sensitive, high resolution measures of cellular metabolism. However, often in vivo measurements of tissue are not feasible. In this study, we investigate the stability over time of two-photon auto-fluorescence imaging of NADH and FAD in live-cultured tissues. Our results demonstrate that cultured tissues remain viable for at least several days post excision. Furthermore, the optical redox ratio, NADH fluorescence lifetime, and FAD fluorescence lifetime do not significantly change in the cultured tissues over time. With these findings, we demonstrate the potential of sustained tissue culture techniques for optical metabolic imaging.",

keywords = "Cancer, Cellular metabolism, Clinical translation, Multi-photon fluorescence",

author = "Walsh, {Alex J.} and Cook, {Rebecca S.} and Arteag, {Carlos L.} and Skala, {Melissa C.}",

year = "2013",

doi = "10.1117/12.2001863",

language = "English (US)",

isbn = "9780819493576",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIII",

note = "Multiphoton Microscopy in the Biomedical Sciences XIII ; Conference date: 03-02-2013 Through 05-02-2013",

}

TY - GEN

T1 - Optical metabolic imaging of live tissue cultures

AU - Walsh, Alex J.

AU - Cook, Rebecca S.

AU - Arteag, Carlos L.

AU - Skala, Melissa C.

PY - 2013

Y1 - 2013

N2 - The fluorescence properties, both intensity and fluorescence lifetime, of NADH and FAD, two coenzymes of metabolism, are sensitive, high resolution measures of cellular metabolism. However, often in vivo measurements of tissue are not feasible. In this study, we investigate the stability over time of two-photon auto-fluorescence imaging of NADH and FAD in live-cultured tissues. Our results demonstrate that cultured tissues remain viable for at least several days post excision. Furthermore, the optical redox ratio, NADH fluorescence lifetime, and FAD fluorescence lifetime do not significantly change in the cultured tissues over time. With these findings, we demonstrate the potential of sustained tissue culture techniques for optical metabolic imaging.

AB - The fluorescence properties, both intensity and fluorescence lifetime, of NADH and FAD, two coenzymes of metabolism, are sensitive, high resolution measures of cellular metabolism. However, often in vivo measurements of tissue are not feasible. In this study, we investigate the stability over time of two-photon auto-fluorescence imaging of NADH and FAD in live-cultured tissues. Our results demonstrate that cultured tissues remain viable for at least several days post excision. Furthermore, the optical redox ratio, NADH fluorescence lifetime, and FAD fluorescence lifetime do not significantly change in the cultured tissues over time. With these findings, we demonstrate the potential of sustained tissue culture techniques for optical metabolic imaging.

KW - Cancer

KW - Cellular metabolism

KW - Clinical translation

KW - Multi-photon fluorescence

UR - http://www.scopus.com/inward/record.url?scp=84878713983&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878713983&partnerID=8YFLogxK

U2 - 10.1117/12.2001863

DO - 10.1117/12.2001863

M3 - Conference contribution

AN - SCOPUS:84878713983

SN - 9780819493576

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Multiphoton Microscopy in the Biomedical Sciences XIII

T2 - Multiphoton Microscopy in the Biomedical Sciences XIII

Y2 - 3 February 2013 through 5 February 2013

ER -

Optical metabolic imaging of live tissue cultures

Abstract

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Keywords

ASJC Scopus subject areas

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