TY - JOUR
T1 - Personalized Hydrogels for Engineering Diverse Fully Autologous Tissue Implants
AU - Edri, Reuven
AU - Gal, Idan
AU - Noor, Nadav
AU - Harel, Tom
AU - Fleischer, Sharon
AU - Adadi, Nofar
AU - Green, Ori
AU - Shabat, Doron
AU - Heller, Lior
AU - Shapira, Assaf
AU - Gat-Viks, Irit
AU - Peer, Dan
AU - Dvir, Tal
N1 - Funding Information:
R.E., I.G., and N.N. contributed equally to this work. R.E. was supported by Tel Aviv University Center for Nanosience and Nanotechnology. T.D. received support from European Research Council Starting Grant 637943, the Slezak Foundation, the Israeli Science Foundation (700/13), the Israel Ministry of Science, Technology and Space (3-12587), and Moxie Foundation. The authors would like to thank Dr. Rivka Ofir from Ben-Gurion University, Israel, for generating and supplying the human omentum iPSCs.
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/1/4
Y1 - 2019/1/4
N2 - Despite incremental improvements in the field of tissue engineering, no technology is currently available for producing completely autologous implants where both the cells and the scaffolding material are generated from the patient, and thus do not provoke an immune response that may lead to implant rejection. Here, a new approach is introduced to efficiently engineer any tissue type, which its differentiation cues are known, from one small tissue biopsy. Pieces of omental tissues are extracted from patients and, while the cells are reprogrammed to become induced pluripotent stem cells, the extracellular matrix is processed into an immunologically matching, thermoresponsive hydrogel. Efficient cell differentiation within a large 3D hydrogel is reported, and, as a proof of concept, the generation of functional cardiac, cortical, spinal cord, and adipogenic tissue implants is demonstrated. This versatile bioengineering approach may assist to regenerate any tissue and organ with a minimal risk for immune rejection.
AB - Despite incremental improvements in the field of tissue engineering, no technology is currently available for producing completely autologous implants where both the cells and the scaffolding material are generated from the patient, and thus do not provoke an immune response that may lead to implant rejection. Here, a new approach is introduced to efficiently engineer any tissue type, which its differentiation cues are known, from one small tissue biopsy. Pieces of omental tissues are extracted from patients and, while the cells are reprogrammed to become induced pluripotent stem cells, the extracellular matrix is processed into an immunologically matching, thermoresponsive hydrogel. Efficient cell differentiation within a large 3D hydrogel is reported, and, as a proof of concept, the generation of functional cardiac, cortical, spinal cord, and adipogenic tissue implants is demonstrated. This versatile bioengineering approach may assist to regenerate any tissue and organ with a minimal risk for immune rejection.
KW - autologous
KW - decellularized hydrogels
KW - induced pluripotent stem cells
KW - non-immunogenic
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85056153402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85056153402&partnerID=8YFLogxK
U2 - 10.1002/adma.201803895
DO - 10.1002/adma.201803895
M3 - Article
C2 - 30406960
AN - SCOPUS:85056153402
SN - 0935-9648
VL - 31
JO - Advanced Materials
JF - Advanced Materials
IS - 1
M1 - 1803895
ER -