Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells

Barbara Adinolfi; Francesco Baldini; Ambra Giannetti; Sara Tombelli; Cosimo Trono; Giovanna Sotgiu; Greta Varchi; Mario Pellegrino; Claudio Domenici

doi:10.1109/NANOFIM.2015.8425357

Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells

Barbara Adinolfi, Francesco Baldini, Ambra Giannetti, Sara Tombelli, Cosimo Trono, Giovanna Sotgiu, Greta Varchi, Mario Pellegrino, Claudio Domenici

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The use of antisense oligonucleotide molecular beacons, able to generate a fluorescent signal when they hybridize with their target mRNA, represents an innovative strategy in cancer. This approach is able to conjugate the ability of sensing specific mRNA with the pharmacological silencing activity, preventing the overexpression of proteins associated to cancer development. In this context, this strategy minimizes the non-specific toxicity addressing the therapy mainly towards the tumor cells by using effective delivery systems. The aim of this work was to investigate the ability of polymethylmethacrylate nanoparticles (PMMA-NPs) to act as vehicle of an oligonucleotide molecular beacon (MB) targeting survivin mRNA in A549 human lung adenocarcinoma epithelial cells. Furthermore, this paper focuses the attention on the need for having an appropriate healthy control in in-vitro experiments. In particular, the survivin-MB was firstly characterized in solution in order to verify its functionality and then the PMMA-NPs ability to promote the MB internalization was verified in A549 cells by confocal microscopy. Confluent Human Dermal Fibroblasts from adult (HDFa) were used as healthy control. The results showed that PMMA-NPs promote the survivin-MB cellular up-take and that the use of 10 μg/mL PMMA-NPs as carrier for survivin-MB for 1h 30 min might be a promising strategy to reduce cancer cell proliferation avoiding detectable consequences on the healthy cells.

Original language	English (US)
Title of host publication	2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	35-38
Number of pages	4
ISBN (Print)	9781509051519
DOIs	https://doi.org/10.1109/NANOFIM.2015.8425357
State	Published - Aug 3 2018
Event	1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015 - Lecce, Italy Duration: Jul 24 2015 → Jul 25 2015

Publication series

Name	2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015

Other

Other	1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015
Country/Territory	Italy
City	Lecce
Period	7/24/15 → 7/25/15

Keywords

cancer cells
fibroblasts
molecular beacon
nanoparticles
survivin

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation

Access to Document

10.1109/NANOFIM.2015.8425357

Cite this

Adinolfi, B., Baldini, F., Giannetti, A., Tombelli, S., Trono, C., Sotgiu, G., Varchi, G., Pellegrino, M., & Domenici, C. (2018). Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells. In 2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015 (pp. 35-38). Article 8425357 (2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NANOFIM.2015.8425357

Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells. / Adinolfi, Barbara; Baldini, Francesco; Giannetti, Ambra et al.
2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015. Institute of Electrical and Electronics Engineers Inc., 2018. p. 35-38 8425357 (2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Adinolfi, B, Baldini, F, Giannetti, A, Tombelli, S, Trono, C, Sotgiu, G, Varchi, G, Pellegrino, M & Domenici, C 2018, Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells. in 2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015., 8425357, 2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015, Institute of Electrical and Electronics Engineers Inc., pp. 35-38, 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015, Lecce, Italy, 7/24/15. https://doi.org/10.1109/NANOFIM.2015.8425357

Adinolfi B, Baldini F, Giannetti A, Tombelli S, Trono C, Sotgiu G et al. Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells. In 2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015. Institute of Electrical and Electronics Engineers Inc. 2018. p. 35-38. 8425357. (2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015). doi: 10.1109/NANOFIM.2015.8425357

Adinolfi, Barbara ; Baldini, Francesco ; Giannetti, Ambra et al. / Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells. 2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 35-38 (2015 1st Workshop on Nanotechnology in Instrumentation and Measurement, NANOFIM 2015).

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abstract = "The use of antisense oligonucleotide molecular beacons, able to generate a fluorescent signal when they hybridize with their target mRNA, represents an innovative strategy in cancer. This approach is able to conjugate the ability of sensing specific mRNA with the pharmacological silencing activity, preventing the overexpression of proteins associated to cancer development. In this context, this strategy minimizes the non-specific toxicity addressing the therapy mainly towards the tumor cells by using effective delivery systems. The aim of this work was to investigate the ability of polymethylmethacrylate nanoparticles (PMMA-NPs) to act as vehicle of an oligonucleotide molecular beacon (MB) targeting survivin mRNA in A549 human lung adenocarcinoma epithelial cells. Furthermore, this paper focuses the attention on the need for having an appropriate healthy control in in-vitro experiments. In particular, the survivin-MB was firstly characterized in solution in order to verify its functionality and then the PMMA-NPs ability to promote the MB internalization was verified in A549 cells by confocal microscopy. Confluent Human Dermal Fibroblasts from adult (HDFa) were used as healthy control. The results showed that PMMA-NPs promote the survivin-MB cellular up-take and that the use of 10 μg/mL PMMA-NPs as carrier for survivin-MB for 1h 30 min might be a promising strategy to reduce cancer cell proliferation avoiding detectable consequences on the healthy cells.",

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AU - Baldini, Francesco

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AU - Tombelli, Sara

AU - Trono, Cosimo

AU - Sotgiu, Giovanna

AU - Varchi, Greta

AU - Pellegrino, Mario

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AB - The use of antisense oligonucleotide molecular beacons, able to generate a fluorescent signal when they hybridize with their target mRNA, represents an innovative strategy in cancer. This approach is able to conjugate the ability of sensing specific mRNA with the pharmacological silencing activity, preventing the overexpression of proteins associated to cancer development. In this context, this strategy minimizes the non-specific toxicity addressing the therapy mainly towards the tumor cells by using effective delivery systems. The aim of this work was to investigate the ability of polymethylmethacrylate nanoparticles (PMMA-NPs) to act as vehicle of an oligonucleotide molecular beacon (MB) targeting survivin mRNA in A549 human lung adenocarcinoma epithelial cells. Furthermore, this paper focuses the attention on the need for having an appropriate healthy control in in-vitro experiments. In particular, the survivin-MB was firstly characterized in solution in order to verify its functionality and then the PMMA-NPs ability to promote the MB internalization was verified in A549 cells by confocal microscopy. Confluent Human Dermal Fibroblasts from adult (HDFa) were used as healthy control. The results showed that PMMA-NPs promote the survivin-MB cellular up-take and that the use of 10 μg/mL PMMA-NPs as carrier for survivin-MB for 1h 30 min might be a promising strategy to reduce cancer cell proliferation avoiding detectable consequences on the healthy cells.

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ER -

Polymethylmethacrylate nanoparticles as vehicle for a molecular beacon specific for survivin mRNA in A549 cells

Abstract

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Keywords

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