@inproceedings{13a464abcd9f47b28ffabf075e732592,
title = "Protease-activated quantum dot probes",
abstract = "We demonstrate a novel quantum dot based probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This probe may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically-degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Peptide cleavage results in release of AuNPs and restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 hours of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.",
keywords = "Collagenase, Fluorescence resonance energy transfer, Gold colloid, Optical contrast agent, Optical quenching, Protease imaging, Proteolytic activity, Quantum dot",
author = "Emmanuel Chang and Jiantang Sun and Miller, {Jordan S.} and Yu, {William W.} and Calvin, {Vicki L.} and West, {Jennifer L.} and Rebekah Drezek",
note = "Funding Information: This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF Award Number EEC-0118007. ; Biophotonics and New Therapy Frontiers ; Conference date: 03-04-2006 Through 05-04-2006",
year = "2006",
doi = "10.1117/12.660485",
language = "English (US)",
isbn = "0819462470",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Biophotonics and New Therapy Frontiers",
}