TY - JOUR
T1 - Protein kinase B/AKT isoform 2 drives migration of human mesenchymal stem cells
AU - Bulj, Zrinka
AU - Duchi, Serena
AU - Bevilacqua, Alessandro
AU - Gherardi, Alessandro
AU - Dozza, Barbara
AU - Piccinini, Filippo
AU - Mariani, Giulia Adalgisa
AU - Lucarelli, Enrico
AU - Giannini, Sandro
AU - Donati, Davide
AU - Marmiroli, Sandra
PY - 2013/1
Y1 - 2013/1
N2 - This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.
AB - This study was designed to investigate the migratory behavior of adult human mesenchymal stem cells (MSC) and the underlying mechanism. Cell migration was assessed by transwell, wound healing and time-lapse in vivo motility assays. Pharmacological inhibitors were used to determine the potential mechanism responsible for cell migration and invasion. The tests that were implemented revealed that MSC were fairly migratory. Protein kinase B (AKT) was strongly activated at the basal level. Through our analyses we demonstrated that pharmacological inactivation of AKT2 but not AKT1 significantly decreased cell migration and invasion. Although preliminary, collectively our results indicate that AKT2 activation plays a critical role in enabling MSC migration.
KW - Cell migration
KW - Mesenchymal stem cells
KW - Phosphoinositide-3 kinase/protein kinase B pathway
KW - Protein kinase B Inhibitors
KW - Wound healing
UR - http://www.scopus.com/inward/record.url?scp=84873657307&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84873657307&partnerID=8YFLogxK
U2 - 10.3892/ijo.2012.1700
DO - 10.3892/ijo.2012.1700
M3 - Article
C2 - 23165443
AN - SCOPUS:84873657307
SN - 1019-6439
VL - 42
SP - 118
EP - 126
JO - International Journal of Oncology
JF - International Journal of Oncology
IS - 1
ER -