TY - JOUR
T1 - Pruni cortex ameliorates skin inflammation possibly through HMGB1-NFκB pathway in house dust mite induced atopic dermatitis NC/Nga transgenic mice
AU - Watanabe, Kenichi
AU - Karuppagounder, Vengadeshprabhu
AU - Arumugam, Somasundaram
AU - Thandavarayan, Rajarajan A.
AU - Pitchaimani, Vigneshwaran
AU - Sreedhar, Remya
AU - Afrin, Rejina
AU - Harima, Meilei
AU - Suzuki, Hiroshi
AU - Suzuki, Kenji
AU - Nakamura, Takashi
AU - Nomoto, Mayumi
AU - Miyashita, Shizuka
AU - Fukumoto, Kyoko
AU - Ueno, Kazuyuki
N1 - Publisher Copyright:
© 2015 JCBN.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Pruni cortex, the bark of Prunus jamasakura Siebold ex koidzumi, has been used in the Japanese systems of machine for many years for its anit-inflammatory, antioxidant and antitussive properties. In this study, we investigated the effect of pruni cortex on atopic dermatitis NC/Nga mouse model. Atopic dermatitis-like lesion was induced by the application of house dust mite extract to the dorsal skin. After induction of atopic dermatitis, pruni cortex aqueous extract (1 g/kg, p.o.) was administered daily for 2 weeks. We evaluated dermatitis severity, histopathological changes and cellular protein expression by Western blotting for nuclear and cytoplasmic high mobility group box 1, receptor for advanced glycation end products, nuclear factor κB, apoptosis and inflammatory markers in the skin of atopic dermatitis mice. The clinical observation confirmed that the dermatitis score was significantly lower when treated with pruni cortex than in the atopic dermatitis group. Similarly pruni cortex inhibited hypertrophy and infiltration of inflammatory cells as identified by histopathology. In addition, pruni cortex significantly inhibited the protein expression of cytoplasmic high mobility group box 1, receptor for advanced glycation end products, nuclear p-nuclear factor kappa B, apoptosis and inflammatory markers. These results indicate that pruni cortex may have therapeutic potential in the treatment of atopic dermatitis by attenuating high mobility group box 1 and inflammation possibly through the nuclear factor kappa B path-way.
AB - Pruni cortex, the bark of Prunus jamasakura Siebold ex koidzumi, has been used in the Japanese systems of machine for many years for its anit-inflammatory, antioxidant and antitussive properties. In this study, we investigated the effect of pruni cortex on atopic dermatitis NC/Nga mouse model. Atopic dermatitis-like lesion was induced by the application of house dust mite extract to the dorsal skin. After induction of atopic dermatitis, pruni cortex aqueous extract (1 g/kg, p.o.) was administered daily for 2 weeks. We evaluated dermatitis severity, histopathological changes and cellular protein expression by Western blotting for nuclear and cytoplasmic high mobility group box 1, receptor for advanced glycation end products, nuclear factor κB, apoptosis and inflammatory markers in the skin of atopic dermatitis mice. The clinical observation confirmed that the dermatitis score was significantly lower when treated with pruni cortex than in the atopic dermatitis group. Similarly pruni cortex inhibited hypertrophy and infiltration of inflammatory cells as identified by histopathology. In addition, pruni cortex significantly inhibited the protein expression of cytoplasmic high mobility group box 1, receptor for advanced glycation end products, nuclear p-nuclear factor kappa B, apoptosis and inflammatory markers. These results indicate that pruni cortex may have therapeutic potential in the treatment of atopic dermatitis by attenuating high mobility group box 1 and inflammation possibly through the nuclear factor kappa B path-way.
KW - Atopic dermatitis
KW - High mobility group box protein 1
KW - Inflammation
KW - Nuclear factor κB
KW - Pruni cortex
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U2 - 10.3164/jcbn.14-75
DO - 10.3164/jcbn.14-75
M3 - Article
AN - SCOPUS:84929623787
SN - 0912-0009
VL - 56
SP - 186
EP - 194
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
IS - 3
ER -