Abstract
Twenty patients were evaluated by quantitative endothelial biomicroscopy and the resulting endothelial cell densities compared with those from a specular microscope. Estimated cell densities by this technique, when compared with the specular microscope, demonstrated a Pearson correlation coefficient of +0.977, an average error of -7% and an absolute error of 12%. There were no errors greater than 26% except for one extremely low cell density of 318 cells/mm2 for which the estimate was 476 cells/mm2. This rapid, inexpensive technique requires counting the number of endothelial cells seen across the horizontal diameter of the 0.2-mm projected spot beam of a standard biomicroscope. From this count and the known spot size the endothelial cell density may be accurately calculated. A simplified four-step technique for performing specular microscopy using only the biomicroscope and a method for accurately measuring the size of the projected slit lamp spot beam are explained.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 33-40 |
| Number of pages | 8 |
| Journal | Ophthalmic Surgery |
| Volume | 14 |
| Issue number | 1 |
| State | Published - Jan 1 1983 |
ASJC Scopus subject areas
- Ophthalmology