Real-time monitoring of lipid transfer between vesicles and hybrid bilayers on Au nanoshells using surface enhanced Raman scattering (SERS)

Janardan Kundu; Carly S Levin; Naomi J. Halas

doi:10.1039/b9nr00063a

Real-time monitoring of lipid transfer between vesicles and hybrid bilayers on Au nanoshells using surface enhanced Raman scattering (SERS)

Janardan Kundu, Carly S Levin, Naomi J. Halas

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

To investigate the dynamics of exchange/transfer of lipids between membranes, we have studied the interaction of donor-deuterated DMPC vesicles with DMPC hybrid bilayers on Au nanoshells using SERS. Experimental data confirm partial lipid exchange/transfer in the outer leaflet of the hybrid bilayer. The kinetics of the exchange/transfer process follows a first order process with a rate constant of 1.3 x 10(-4) s(-1). Changes in lipid phase behavior caused by the exchange/transfer process were characterized using generalized polarization measurements. In situ lipid transfer can potentially be utilized for preparation of asymmetric supported lipid bilayers and for incorporation of reporter lipids in biological membranes.

Original language	English (US)
Pages (from-to)	114-7
Number of pages	4
Journal	Nanoscale
Volume	1
Issue number	1
DOIs	https://doi.org/10.1039/b9nr00063a
State	Published - Oct 2009

Keywords

Deuterium Exchange Measurement
Dimyristoylphosphatidylcholine
Gold
Kinetics
Lipid Bilayers
Models, Molecular
Nanoshells
Spectrum Analysis, Raman
Surface Properties
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

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title = "Real-time monitoring of lipid transfer between vesicles and hybrid bilayers on Au nanoshells using surface enhanced Raman scattering (SERS)",

abstract = "To investigate the dynamics of exchange/transfer of lipids between membranes, we have studied the interaction of donor-deuterated DMPC vesicles with DMPC hybrid bilayers on Au nanoshells using SERS. Experimental data confirm partial lipid exchange/transfer in the outer leaflet of the hybrid bilayer. The kinetics of the exchange/transfer process follows a first order process with a rate constant of 1.3 x 10(-4) s(-1). Changes in lipid phase behavior caused by the exchange/transfer process were characterized using generalized polarization measurements. In situ lipid transfer can potentially be utilized for preparation of asymmetric supported lipid bilayers and for incorporation of reporter lipids in biological membranes.",

keywords = "Deuterium Exchange Measurement, Dimyristoylphosphatidylcholine, Gold, Kinetics, Lipid Bilayers, Models, Molecular, Nanoshells, Spectrum Analysis, Raman, Surface Properties, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.",

author = "Janardan Kundu and Levin, {Carly S} and Halas, {Naomi J.}",

year = "2009",

month = oct,

doi = "10.1039/b9nr00063a",

language = "English (US)",

volume = "1",

pages = "114--7",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

number = "1",

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TY - JOUR

T1 - Real-time monitoring of lipid transfer between vesicles and hybrid bilayers on Au nanoshells using surface enhanced Raman scattering (SERS)

AU - Kundu, Janardan

AU - Levin, Carly S

AU - Halas, Naomi J.

PY - 2009/10

Y1 - 2009/10

N2 - To investigate the dynamics of exchange/transfer of lipids between membranes, we have studied the interaction of donor-deuterated DMPC vesicles with DMPC hybrid bilayers on Au nanoshells using SERS. Experimental data confirm partial lipid exchange/transfer in the outer leaflet of the hybrid bilayer. The kinetics of the exchange/transfer process follows a first order process with a rate constant of 1.3 x 10(-4) s(-1). Changes in lipid phase behavior caused by the exchange/transfer process were characterized using generalized polarization measurements. In situ lipid transfer can potentially be utilized for preparation of asymmetric supported lipid bilayers and for incorporation of reporter lipids in biological membranes.

AB - To investigate the dynamics of exchange/transfer of lipids between membranes, we have studied the interaction of donor-deuterated DMPC vesicles with DMPC hybrid bilayers on Au nanoshells using SERS. Experimental data confirm partial lipid exchange/transfer in the outer leaflet of the hybrid bilayer. The kinetics of the exchange/transfer process follows a first order process with a rate constant of 1.3 x 10(-4) s(-1). Changes in lipid phase behavior caused by the exchange/transfer process were characterized using generalized polarization measurements. In situ lipid transfer can potentially be utilized for preparation of asymmetric supported lipid bilayers and for incorporation of reporter lipids in biological membranes.

KW - Deuterium Exchange Measurement

KW - Dimyristoylphosphatidylcholine

KW - Gold

KW - Kinetics

KW - Lipid Bilayers

KW - Models, Molecular

KW - Nanoshells

KW - Spectrum Analysis, Raman

KW - Surface Properties

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.1039/b9nr00063a

DO - 10.1039/b9nr00063a

M3 - Article

C2 - 20644868

SN - 2040-3364

VL - 1

SP - 114

EP - 117

JO - Nanoscale

JF - Nanoscale

IS - 1

ER -

Real-time monitoring of lipid transfer between vesicles and hybrid bilayers on Au nanoshells using surface enhanced Raman scattering (SERS)

Abstract

Keywords

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