TY - JOUR
T1 - Relation between digitalis binding in vivo and inhibition of sodium, potassium-adenosine triphosphatase in canine kidney
AU - Allen, Julius C.
AU - Martinez-Maldonado, M.
AU - Eknoyan, G.
AU - Suki, Wadi N.
AU - Schwartz, Arnold
N1 - Funding Information:
* Supported by USPHS Grants HE 0543590, P.8, NSF GB 6895, HE 43042-01, HE 07906-07 and Veterans Administration Section Grant, 101.122 Project 6848. t Postdoctoral Fellow, USPHS, National Heart Institute (l-F02-HE43042-01). $ Career Research Development Awardee, National Heart Institute (K, HE 11, 875-05). 8 Abbreviations: Na+,K+-ATPase, sodium, potassium-adenosine triphosphatase; C,,, sodium clearance; C..,, osmolar clearance; TCHIO, free water reabsorption; PK, pyruvate kinase; LDH, lactate dehydrogenase; DOC, Na-deoxycholate; PEP, phosphoenol pyruvic acid. 73
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1971/1
Y1 - 1971/1
N2 - Digoxin was infused into a dog renal artery, using the contralateral kidney as control. At peak diuretic effect, sodium, potassium-adenosine triphosphatase (Na+,K+-ATPase) activity of both experimental cortex and medulla was significantly depressed compared to control. The inhibitory effect in vitro of ouabain on the control Na+,K+-ATPase from both medulla and cortex was time dependent; no regional difference in sensitivity was noted. The binding in vitro of 3H-ouabain to Na+, K+-ATPase from both experimental cortex and experimental medulla was significantly lower than that for control enzymes, suggesting previous occupation of drug-binding sites. The data represent further indication that digoxin-induced diuresis is related to inhibition of Na+,K+-ATPase.
AB - Digoxin was infused into a dog renal artery, using the contralateral kidney as control. At peak diuretic effect, sodium, potassium-adenosine triphosphatase (Na+,K+-ATPase) activity of both experimental cortex and medulla was significantly depressed compared to control. The inhibitory effect in vitro of ouabain on the control Na+,K+-ATPase from both medulla and cortex was time dependent; no regional difference in sensitivity was noted. The binding in vitro of 3H-ouabain to Na+, K+-ATPase from both experimental cortex and experimental medulla was significantly lower than that for control enzymes, suggesting previous occupation of drug-binding sites. The data represent further indication that digoxin-induced diuresis is related to inhibition of Na+,K+-ATPase.
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U2 - 10.1016/0006-2952(71)90473-4
DO - 10.1016/0006-2952(71)90473-4
M3 - Article
C2 - 4255110
AN - SCOPUS:0014999133
SN - 0006-2952
VL - 20
SP - 73
EP - 80
JO - Biochemical pharmacology
JF - Biochemical pharmacology
IS - 1
ER -