Abstract
We present a valveless microfluidic device for single cell capture and on-chip whole genome amplification of immobilized DNA. This device uses a series of closely spaced micropillars to physically entrap cells and its genomic DNA (gDNA) upon cell lysis. Our past work has demonstrated high efficiency DNA capture using micropillar arrays [1]. Here, we demonstrate the ability to perform in-channel chemistries, such as DNA amplification on single cells. We report that out of six gene loci sampled, we detect all six loci from amplified product collected from our device compared to two out of six in control samples.
| Original language | English (US) |
|---|---|
| Title of host publication | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1231-1232 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780692941836 |
| State | Published - Jan 1 2020 |
| Event | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States Duration: Oct 22 2017 → Oct 26 2017 |
Other
| Other | 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 |
|---|---|
| Country/Territory | United States |
| City | Savannah |
| Period | 10/22/17 → 10/26/17 |
Keywords
- Amplification Bias
- DNA
- MDA
- Microfluidics
- Single Cell
- Whole Genome Amplification
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering