TY - JOUR
T1 - Spatially resolved in vivo imaging of inflammation-associated mRNA via enzymatic fluorescence amplification in a molecular beacon
AU - Sheng, Chuangui
AU - Zhao, Jian
AU - Di, Zhenghan
AU - Huang, Yuanyu
AU - Zhao, Yuliang
AU - Li, Lele
N1 - Funding Information:
This work was supported financially by the National Natural Science Foundation of China (22125402 (to L.L.) and 22004023 (to J.Z.)), the Youth Innovation Promotion Association CAS (to J.Z.), the National Key R&D Program of China (2021YFA1200104, to L.L.) and the Strategic Priority Research Program of Chinese Academy of Sciences (XDB36000000, to L.L. and Y.Z.).
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2022/9
Y1 - 2022/9
N2 - The in vivo optical imaging of RNA biomarkers of inflammation is hindered by low signal-to-background ratios, owing to non-specific signal amplification in healthy tissues. Here we report the design and in vivo applicability, for the imaging of inflammation-associated messenger RNAs (mRNAs), of a molecular beacon bearing apurinic/apyrimidinic sites, whose amplification of fluorescence is triggered by human apurinic/apyrimidinic endonuclease 1 on translocation from the nucleus into the cytoplasm specifically in inflammatory cells. We assessed the sensitivity and tissue specificity of an engineered molecular beacon targeting interleukin-6 (IL-6) mRNA in live mice, by detecting acute inflammation in their paws and drug-induced inflammation in their livers. This enzymatic-amplification strategy may enable the specific and sensitive imaging of other disease-relevant RNAs in vivo.
AB - The in vivo optical imaging of RNA biomarkers of inflammation is hindered by low signal-to-background ratios, owing to non-specific signal amplification in healthy tissues. Here we report the design and in vivo applicability, for the imaging of inflammation-associated messenger RNAs (mRNAs), of a molecular beacon bearing apurinic/apyrimidinic sites, whose amplification of fluorescence is triggered by human apurinic/apyrimidinic endonuclease 1 on translocation from the nucleus into the cytoplasm specifically in inflammatory cells. We assessed the sensitivity and tissue specificity of an engineered molecular beacon targeting interleukin-6 (IL-6) mRNA in live mice, by detecting acute inflammation in their paws and drug-induced inflammation in their livers. This enzymatic-amplification strategy may enable the specific and sensitive imaging of other disease-relevant RNAs in vivo.
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U2 - 10.1038/s41551-022-00932-z
DO - 10.1038/s41551-022-00932-z
M3 - Article
C2 - 36050523
AN - SCOPUS:85137013170
SN - 2157-846X
VL - 6
SP - 1074
EP - 1084
JO - Nature Biomedical Engineering
JF - Nature Biomedical Engineering
IS - 9
ER -