TY - JOUR
T1 - Stem cell Janus patch for periodontal regeneration
AU - Yan, Na
AU - Hu, Bin
AU - Xu, Junchao
AU - Cai, Rong
AU - Liu, Zhenhai
AU - Fu, Dapeng
AU - Huo, Beibei
AU - Liu, Zhenhua
AU - Zhao, Yuliang
AU - Chen, Chunying
AU - Xu, Weihua
N1 - Funding Information:
We thank Jishuitan Hospital and Beijing Zhongguancun Hospital for providing human dental-derived stem cells for this experiment and for helping with the animal experiments. We thank the Institute of Process Engineering for providing the CT equipment for post-animal experimental evaluation. This work was financially supported by the Ministry of Science and Technology of China (2016YFA0201600) and the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB36000000).
Funding Information:
We thank Jishuitan Hospital and Beijing Zhongguancun Hospital for providing human dental-derived stem cells for this experiment and for helping with the animal experiments. We thank the Institute of Process Engineering for providing the CT equipment for post-animal experimental evaluation. This work was financially supported by the Ministry of Science and Technology of China ( 2016YFA0201600 ) and the Strategic Priority Research Program of the Chinese Academy of Sciences ( XDB36000000 ).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2022/2
Y1 - 2022/2
N2 - Periodontitis places a substantial burden on healthcare worldwide. Due to the complexity of the periodontium, regenerative periodontal therapy in healing periodontal tissue damage caused by periodontitis has long been difficult using traditional tissue engineering techniques. The current clinical treatment, guided tissue regeneration (GTR), has shown limitations in reconstructing an osteogenic microenvironment. Here, we designed a stem cell-containing patch (stem cell “Janus” patch; SCJP) to acclimatize stem cells in the osteogenic and odontogenic microenvironment for periodontal regeneration. The SCJP promoted periodontal regeneration within its functional layer, while its barrier layer blocked gingival epithelial cells (GECs) invasion. Specifically, embedded dental pulp stem cells (DPSCs) in the functional layer facilitated the regeneration of dentin, cementum and alveolar bone, all of which orchestrated complete periodontal structure regeneration. We characterized the biophysical properties of the SCJP barrier layer, which has a compact structure and appreciable resistance to mechanical stress, suggesting the patch may be effective in preventing epithelium invasion. Successful ectopic mineralization and further osteogenic and odontogenic differentiation in mouse, rat and swine periodontal defect models further validated the patch's potential in accelerating periodontal tissue regeneration. Our study describes a safe and effective stem cell-base strategy for a therapeutic that not only realizes periodontal repair but also protects the restoring periodontium from epithelial cell invasion.
AB - Periodontitis places a substantial burden on healthcare worldwide. Due to the complexity of the periodontium, regenerative periodontal therapy in healing periodontal tissue damage caused by periodontitis has long been difficult using traditional tissue engineering techniques. The current clinical treatment, guided tissue regeneration (GTR), has shown limitations in reconstructing an osteogenic microenvironment. Here, we designed a stem cell-containing patch (stem cell “Janus” patch; SCJP) to acclimatize stem cells in the osteogenic and odontogenic microenvironment for periodontal regeneration. The SCJP promoted periodontal regeneration within its functional layer, while its barrier layer blocked gingival epithelial cells (GECs) invasion. Specifically, embedded dental pulp stem cells (DPSCs) in the functional layer facilitated the regeneration of dentin, cementum and alveolar bone, all of which orchestrated complete periodontal structure regeneration. We characterized the biophysical properties of the SCJP barrier layer, which has a compact structure and appreciable resistance to mechanical stress, suggesting the patch may be effective in preventing epithelium invasion. Successful ectopic mineralization and further osteogenic and odontogenic differentiation in mouse, rat and swine periodontal defect models further validated the patch's potential in accelerating periodontal tissue regeneration. Our study describes a safe and effective stem cell-base strategy for a therapeutic that not only realizes periodontal repair but also protects the restoring periodontium from epithelial cell invasion.
KW - Dental pulp stem cell
KW - Guided tissue regeneration
KW - Osteogenic differentiation
KW - Tissue engineering
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U2 - 10.1016/j.nantod.2021.101336
DO - 10.1016/j.nantod.2021.101336
M3 - Article
AN - SCOPUS:85118999389
SN - 1748-0132
VL - 42
JO - Nano Today
JF - Nano Today
M1 - 101336
ER -