TY - JOUR
T1 - Studies on the Metabolism of C19‐Steroids in Rat Liver
T2 - 1. Hydroxylation of Testosterone in Rat Liver Microsomes
AU - LISBOA, B. P.
AU - GUSTAFSSON, J.
AU - SJÖVALL, J.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1968/5
Y1 - 1968/5
N2 - The formation of polar C19‐steroids with a 3‐oxo‐Δ4‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.
AB - The formation of polar C19‐steroids with a 3‐oxo‐Δ4‐structure after incubation of testosterone with 105,000 ×g microsomes of adult male rat liver was investigated. The ultraviolet‐positive metabolites were isolated by thin‐layer chromatography. The isolated compounds were identified as 2β‐, 6β‐, 7α‐, and 16α‐hydroxytestosterone by means of gas‐liquid chromatography‐mass spectrometry. A dihydroxytesterone was also isolated and tentatively identified as 6β,16α,17β‐tri‐hydroxyandrost‐4‐en‐3‐one.
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U2 - 10.1111/j.1432-1033.1968.tb00240.x
DO - 10.1111/j.1432-1033.1968.tb00240.x
M3 - Article
C2 - 5660482
AN - SCOPUS:0014280482
SN - 0014-2956
VL - 4
SP - 496
EP - 505
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 4
ER -