Abstract
Mammalian SVZ progenitors continuously generate new neurons in the olfactory bulb. After injury, changes in SVZ cell number suggest injury-induced migration. Studies that trace the migration of SVZ precursors into neurodegenerating areas are lacking. Previously, we showed a decrease in BrdU+SVZ cells following excitotoxic damage to the immature rat cortex. Here, we demonstrate that NMDA-induced injury forces endogenous Cell Tracker Green (CTG) labeled VZ/SVZ precursors out of the SVZ into the neurodegenerating cortex. CTG+/Nestin+/Filamin A+ precursors are closely associated with vimentin+/GFAP+/GLAST+ filaments and express both chemokine receptor CXCR4 and Robo1. In the cortex, SVZ-derived progenitors show a progressive expression of developing, migrating and mature neurons and glial markers. CTG+/GFAP+ astrocytes greatly outnumber CTG+/MAP2+/NeuN+ neurons. SVZ-derived progenitors differentiate into both tbr1+ cortical glutamatergic neurons and calretinin+ interneurons. But, there is little integration of these neurons into the existing circuitry, as seen by Fluorogold retrograde tracing from the internal capsule.
Original language | English (US) |
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Pages (from-to) | 170-82 |
Number of pages | 13 |
Journal | Molecular and Cellular Neuroscience |
Volume | 38 |
Issue number | 2 |
DOIs | |
State | Published - Jun 2008 |
Keywords
- Age Factors
- Animals
- Astrocytes
- Biomarkers
- Calbindin 2
- Cell Differentiation
- Cell Division
- Cell Movement
- Excitatory Amino Acid Agonists
- Female
- Gliosis
- Internal Capsule
- Male
- Motor Cortex
- N-Methylaspartate
- Nerve Degeneration
- Nerve Tissue Proteins
- Neurons
- Neurotoxins
- Rats
- Rats, Long-Evans
- Receptors, CXCR4
- Receptors, Immunologic
- S100 Calcium Binding Protein G
- Somatosensory Cortex
- Stem Cells
- Journal Article
- Research Support, Non-U.S. Gov't