TY - CHAP
T1 - T2 magnetic resonance for fungal diagnosis
AU - Zervou, Fainareti N.
AU - Zacharioudakis, Ioannis M.
AU - Kurpewski, Jaclynn
AU - Mylonakis, Eleftherios
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2017.
PY - 2017
Y1 - 2017
N2 - Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic platform that was recently approved by the US Food and Drug Administration (FDA) for diagnosis of candidemia with a mean time to species identification of less than 5 h. T2Candida panel is performed on the fully automated T2Dx instrument in whole blood K2 EDTA specimens and is able to detect 5 Candida spp., namely Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, and Candida glabrata. By combining magnetic resonance with molecular diagnostics, T2 Candida panel amplifies DNA and detects the amplified product by amplicon-induced agglomeration of supermagnetic particles and T2 Magnetic Resonance (T2MR) measurement. Here we describe the materials and methods needed to diagnose candidemia with the T2Candida panel.
AB - Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic platform that was recently approved by the US Food and Drug Administration (FDA) for diagnosis of candidemia with a mean time to species identification of less than 5 h. T2Candida panel is performed on the fully automated T2Dx instrument in whole blood K2 EDTA specimens and is able to detect 5 Candida spp., namely Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, and Candida glabrata. By combining magnetic resonance with molecular diagnostics, T2 Candida panel amplifies DNA and detects the amplified product by amplicon-induced agglomeration of supermagnetic particles and T2 Magnetic Resonance (T2MR) measurement. Here we describe the materials and methods needed to diagnose candidemia with the T2Candida panel.
KW - Candida
KW - T2 Candida panel
KW - T2 magnetic resonance
KW - T2MR
UR - http://www.scopus.com/inward/record.url?scp=84995511594&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84995511594&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-6515-1_18
DO - 10.1007/978-1-4939-6515-1_18
M3 - Chapter
C2 - 27837513
AN - SCOPUS:84995511594
T3 - Methods in Molecular Biology
SP - 305
EP - 319
BT - Methods in Molecular Biology
PB - Humana Press
ER -