The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

Hosam M. Al-Jehani; Alaa N. Turkistani; Majd M. Alrayes; May A. AlHamid; Arwa S. AlShamikh; Zhra M. Al Sadah; Faisal M. Alabbas; Cyril Cyrus; Amein K. Al-Ali

doi:10.17712/nsj.2023.4.20230028

The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

Hosam M. Al-Jehani, Alaa N. Turkistani, Majd M. Alrayes, May A. AlHamid, Arwa S. AlShamikh, Zhra M. Al Sadah, Faisal M. Alabbas, Cyril Cyrus, Amein K. Al-Ali

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVES: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.

METHODS: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.

RESULTS: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls ( p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not.

CONCLUSION: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.

Original language	English (US)
Pages (from-to)	250-257
Number of pages	8
Journal	Neurosciences
Volume	28
Issue number	4
DOIs	https://doi.org/10.17712/nsj.2023.4.20230028
State	Published - Oct 1 2023

Keywords

Humans
MicroRNAs/genetics
Intracranial Aneurysm/diagnostic imaging
Prognosis
Case-Control Studies
Biomarkers

ASJC Scopus subject areas

Clinical Neurology
Psychiatry and Mental health

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10.17712/nsj.2023.4.20230028

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@article{737c57a3b86944d89e468a97b0cfdb3f,

title = "The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms",

abstract = "OBJECTIVES: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.METHODS: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.RESULTS: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls ( p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not. CONCLUSION: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.",

keywords = "Humans, MicroRNAs/genetics, Intracranial Aneurysm/diagnostic imaging, Prognosis, Case-Control Studies, Biomarkers",

author = "Al-Jehani, {Hosam M.} and Turkistani, {Alaa N.} and Alrayes, {Majd M.} and AlHamid, {May A.} and AlShamikh, {Arwa S.} and {Al Sadah}, {Zhra M.} and Alabbas, {Faisal M.} and Cyril Cyrus and Al-Ali, {Amein K.}",

note = "Copyright: {\textcopyright} Neurosciences.",

year = "2023",

month = oct,

day = "1",

doi = "10.17712/nsj.2023.4.20230028",

language = "English (US)",

volume = "28",

pages = "250--257",

journal = "Neurosciences",

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publisher = "Saudi Arabian Armed Forces Hospital",

number = "4",

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TY - JOUR

T1 - The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

AU - Al-Jehani, Hosam M.

AU - Turkistani, Alaa N.

AU - Alrayes, Majd M.

AU - AlHamid, May A.

AU - AlShamikh, Arwa S.

AU - Al Sadah, Zhra M.

AU - Alabbas, Faisal M.

AU - Cyrus, Cyril

AU - Al-Ali, Amein K.

N1 - Copyright: © Neurosciences.

PY - 2023/10/1

Y1 - 2023/10/1

N2 - OBJECTIVES: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.METHODS: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.RESULTS: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls ( p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not. CONCLUSION: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.

AB - OBJECTIVES: To identify the dysregulated functional miRNAs, miRNA-16, miRNA-143, and miRNA-200 as potential biomarkers of cerebral aneurysms(CAs) to aid in diagnosis and prognosis.METHODS: This is a prospective case-control study conducted among patients with CA. All patients' computed tomography angiography (CTA) and interventional angiogram were assessed and studied. The miRNAs were isolated and quantified from peripheral blood samples and the expression profiling was done using TaqMan chemistry on Real-Time PCR.RESULTS: A total of 37 samples were included. Three cases had double aneurysms and 10 cases presented with aneurysmal rupture. miRNA-16, miRNA-143, and miRNA-200 were upregulated with an absolute fold-change of >8 in the CA group in comparison to controls ( p<0.05). miRNA-200 showed double expression in patients with single aneurysm. A statically significant increase was observed in the expression of miRNA-143 in patients who had an aneurysmal rupture with p<0.05. Diabetic patients showed an obvious increase in miRNA-200 (13.03 folds) and miRNA-16 (26.82 folds) expression. Also, there was a notable elevation in miRNA-16, miRNA-143, and miRNA-200 in patients who had hypertension in comparison to those who did not. CONCLUSION: miRNA-16, miRNA-143, and miRNA-200 showed statically significant higher expression among cases with cerebral aneurysms in comparison to controls. Thus, these preliminary results of miRNAs biomarkers are promising future tool to be used for aneurysmal screening.

KW - Humans

KW - MicroRNAs/genetics

KW - Intracranial Aneurysm/diagnostic imaging

KW - Prognosis

KW - Case-Control Studies

KW - Biomarkers

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DO - 10.17712/nsj.2023.4.20230028

M3 - Article

C2 - 37844954

AN - SCOPUS:85175741435

SN - 1319-6138

VL - 28

SP - 250

EP - 257

JO - Neurosciences

JF - Neurosciences

IS - 4

ER -

The role of MicroRNA as a potential diagnostic and prognostic biomarker for cerebral aneurysms

Abstract

Keywords

ASJC Scopus subject areas

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