TY - JOUR
T1 - Toluidine blue and hematoxylin and eosin stains are comparable in evaluating squamous cell carcinoma during mohs
AU - Styperek, Andrew R.
AU - Goldberg, Leonard H.
AU - Goldschmidt, Laura E.
AU - Kimyai-Asadi, Arash
PY - 2016/11/1
Y1 - 2016/11/1
N2 - BACKGROUND Histologic examination of tissue is the foundation of Mohs micrographic surgery because determination of surgical margins influences whether additional tissue will be taken. Currently, there is no large focused study comparing toluidine blue (TB) and hematoxylin and eosin (H&E) stains in the evaluation of squamous cell carcinoma (SCC). OBJECTIVE This study evaluates whether TB and H&E are comparable in assessing the presence of tumor in frozen sections of SCC. MATERIALS AND METHODS One hundred eighty-six randomized slides representing 93 tissue pieces from 36 tumors were examined by 3 Mohs surgeons (1 Accreditation Council for Graduate Medical Education fellow and 2 fellowship-trained surgeons) and compared using a template that documented the presence and location of tumor on the slides. RESULTS The evaluation of SCC with H&E and TB stains was highly concordant, with concordant identification of SCC in 96%, 96%, and 94% of tissue layers among the 3 Mohs surgeons ARS, LHG, and AK-A, respectively. CONCLUSION Toluidine blue and H&E stains are statistically similar in their ability to detect SCC and guide Mohs surgical decision-making.
AB - BACKGROUND Histologic examination of tissue is the foundation of Mohs micrographic surgery because determination of surgical margins influences whether additional tissue will be taken. Currently, there is no large focused study comparing toluidine blue (TB) and hematoxylin and eosin (H&E) stains in the evaluation of squamous cell carcinoma (SCC). OBJECTIVE This study evaluates whether TB and H&E are comparable in assessing the presence of tumor in frozen sections of SCC. MATERIALS AND METHODS One hundred eighty-six randomized slides representing 93 tissue pieces from 36 tumors were examined by 3 Mohs surgeons (1 Accreditation Council for Graduate Medical Education fellow and 2 fellowship-trained surgeons) and compared using a template that documented the presence and location of tumor on the slides. RESULTS The evaluation of SCC with H&E and TB stains was highly concordant, with concordant identification of SCC in 96%, 96%, and 94% of tissue layers among the 3 Mohs surgeons ARS, LHG, and AK-A, respectively. CONCLUSION Toluidine blue and H&E stains are statistically similar in their ability to detect SCC and guide Mohs surgical decision-making.
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U2 - 10.1097/DSS.0000000000000872
DO - 10.1097/DSS.0000000000000872
M3 - Article
C2 - 27662051
AN - SCOPUS:84994684125
SN - 1076-0512
VL - 42
SP - 1279
EP - 1284
JO - Dermatologic Surgery
JF - Dermatologic Surgery
IS - 11
ER -