TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol

Zhiyu Zhang; Zejuan Li; Xiaohui Wu; Chun Feng Zhang; Tyler Calway; Tong Chuan He; Wei Du; Jianjun Chen; Chong Zhi Wang; Chun Su Yuan

doi:10.1016/j.jphs.2014.11.003

TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol

Zhiyu Zhang, Zejuan Li, Xiaohui Wu, Chun Feng Zhang, Tyler Calway, Tong Chuan He, Wei Du, Jianjun Chen, Chong Zhi Wang, Chun Su Yuan

Houston Methodist

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Among important components of American ginseng, protopanaxadiol (PPD) showed more active anticancer potential than other triterpenoid saponins. In this study, we determined the in vivo effects of PPD in a mouse cancer model first. Then, using human colorectal cancer cell lines, we observed significant cancer cell growth inhibition by promoting G1 cell cycle redistribution and apoptosis. Subsequently, we characterized the downstream genes targeted by PPD in HCT-116 cancer cells. Using Affymetrix high density GeneChips, we obtained the gene expression profile of the cells. Microarray data indicated that the expression levels of 76 genes were changed over two-fold after PPD, of which 52 were upregulated while the remaining 24 were downregulated. Ingenuity pathway analysis of top functions affected was carried out. Data suggested that by regulating the interactions between p53 and DR4/DR5, the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway played a key role in the action of PPD, a promising colon cancer inhibitory compound.

Original language	English (US)
Pages (from-to)	83-91
Number of pages	9
Journal	Journal of Pharmacological Sciences
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/j.jphs.2014.11.003
State	Published - Jan 2015

Keywords

Apoptosis
Gene expression
Human colorectal cancer
Microarray
Panax quinquefolius
Protopanaxadiol
Tumor necrosis factor
Xenograft

ASJC Scopus subject areas

Molecular Medicine
Pharmacology

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10.1016/j.jphs.2014.11.003

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title = "TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol",

abstract = "Among important components of American ginseng, protopanaxadiol (PPD) showed more active anticancer potential than other triterpenoid saponins. In this study, we determined the in vivo effects of PPD in a mouse cancer model first. Then, using human colorectal cancer cell lines, we observed significant cancer cell growth inhibition by promoting G1 cell cycle redistribution and apoptosis. Subsequently, we characterized the downstream genes targeted by PPD in HCT-116 cancer cells. Using Affymetrix high density GeneChips, we obtained the gene expression profile of the cells. Microarray data indicated that the expression levels of 76 genes were changed over two-fold after PPD, of which 52 were upregulated while the remaining 24 were downregulated. Ingenuity pathway analysis of top functions affected was carried out. Data suggested that by regulating the interactions between p53 and DR4/DR5, the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway played a key role in the action of PPD, a promising colon cancer inhibitory compound.",

keywords = "Apoptosis, Gene expression, Human colorectal cancer, Microarray, Panax quinquefolius, Protopanaxadiol, Tumor necrosis factor, Xenograft",

author = "Zhiyu Zhang and Zejuan Li and Xiaohui Wu and Zhang, {Chun Feng} and Tyler Calway and He, {Tong Chuan} and Wei Du and Jianjun Chen and Wang, {Chong Zhi} and Yuan, {Chun Su}",

note = "Funding Information: This work was supported in part by the grants of NIH/ NCCAM AT004418 and AT005362 , NIH/ NIGMS 074197 and 5P30DK042086 , NIH/ NCI CA149275 , and U.S. DOD W81XWH-10-1-0077 Publisher Copyright: {\textcopyright} 2014 Japanese Pharmacological Society.",

year = "2015",

month = jan,

doi = "10.1016/j.jphs.2014.11.003",

language = "English (US)",

volume = "127",

pages = "83--91",

journal = "Journal of Pharmacological Sciences",

issn = "1347-8613",

publisher = "Japanese Pharmacological Society",

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TY - JOUR

T1 - TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol

AU - Zhang, Zhiyu

AU - Li, Zejuan

AU - Wu, Xiaohui

AU - Zhang, Chun Feng

AU - Calway, Tyler

AU - He, Tong Chuan

AU - Du, Wei

AU - Chen, Jianjun

AU - Wang, Chong Zhi

AU - Yuan, Chun Su

N1 - Funding Information: This work was supported in part by the grants of NIH/ NCCAM AT004418 and AT005362 , NIH/ NIGMS 074197 and 5P30DK042086 , NIH/ NCI CA149275 , and U.S. DOD W81XWH-10-1-0077 Publisher Copyright: © 2014 Japanese Pharmacological Society.

PY - 2015/1

Y1 - 2015/1

N2 - Among important components of American ginseng, protopanaxadiol (PPD) showed more active anticancer potential than other triterpenoid saponins. In this study, we determined the in vivo effects of PPD in a mouse cancer model first. Then, using human colorectal cancer cell lines, we observed significant cancer cell growth inhibition by promoting G1 cell cycle redistribution and apoptosis. Subsequently, we characterized the downstream genes targeted by PPD in HCT-116 cancer cells. Using Affymetrix high density GeneChips, we obtained the gene expression profile of the cells. Microarray data indicated that the expression levels of 76 genes were changed over two-fold after PPD, of which 52 were upregulated while the remaining 24 were downregulated. Ingenuity pathway analysis of top functions affected was carried out. Data suggested that by regulating the interactions between p53 and DR4/DR5, the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway played a key role in the action of PPD, a promising colon cancer inhibitory compound.

AB - Among important components of American ginseng, protopanaxadiol (PPD) showed more active anticancer potential than other triterpenoid saponins. In this study, we determined the in vivo effects of PPD in a mouse cancer model first. Then, using human colorectal cancer cell lines, we observed significant cancer cell growth inhibition by promoting G1 cell cycle redistribution and apoptosis. Subsequently, we characterized the downstream genes targeted by PPD in HCT-116 cancer cells. Using Affymetrix high density GeneChips, we obtained the gene expression profile of the cells. Microarray data indicated that the expression levels of 76 genes were changed over two-fold after PPD, of which 52 were upregulated while the remaining 24 were downregulated. Ingenuity pathway analysis of top functions affected was carried out. Data suggested that by regulating the interactions between p53 and DR4/DR5, the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) pathway played a key role in the action of PPD, a promising colon cancer inhibitory compound.

KW - Apoptosis

KW - Gene expression

KW - Human colorectal cancer

KW - Microarray

KW - Panax quinquefolius

KW - Protopanaxadiol

KW - Tumor necrosis factor

KW - Xenograft

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TRAIL pathway is associated with inhibition of colon cancer by protopanaxadiol

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